摘要
探讨TCTE3 mRNA在人肝癌细胞株中的表达水平及意义。采用实时荧光定量PCR技术分别检测TCTE3基因在常规培养或缺氧诱导(CoCl2为诱导剂)的人肝癌细胞株中的表达情况。TCTE3 mRNA在常规培养的人正常肝细胞株L02,肝癌细胞株Bel-7402、SMMC-7721、HepG2和QGY-7701中不表达或极低表达;但经缺氧诱导后,SMMC-7721细胞中的TCTE3mRNA表达量逐渐升高,4h达到最大,随后逐渐降低,与HIF1a mRNA表达的曲线变化有一定相似之处,只是这种变化的时间要早于HIF1a基因。在缺氧状态下肝癌细胞TCTE3 mRNA表达增加,且这种上调表达可能对HIF1a mRNA的表达有一定的促进作用。
To investigate genes expression and significance of TCTE3 mRNA in liver cancer cells cultured. TCTE3 mRNA were examined in liver cancer cells under conventional culture or hypoxia-induced culture by CoCl2 by real-time quantitative Polymerase chain reaction （PCR）. No expression or low expression of TCTE3 mRNA levels in normal human liver L02 cells, and liver cancer Bel-7402, SMMC-7721, HepG2 or QGY-7701 cells. However, After SMMC-7721 cells were incubated with hypoxia-inducible CoCl2, the expression of TCTE3 mRNA began to increase gradually, peaked at 4h, and then decreased, which has some similarities with HIF1a mRNA expression curve, but such changes earlier than HIF1a mRNA. The expression of TCTE3 mRNA levels increased, which could promote HIF1a mRNA expression.
出处
《大众科技》
2013年第11期105-107,共3页
Popular Science & Technology
基金
广西卫生厅中医药科技专项(No.GZKZ10-53)
广西教育厅科研项目(No.201010LX262)
