中国汉族人群载脂蛋白M基因-778T/C多态与2型糖尿病合并冠心病的关联研究

Association between apolipoprotein M gene-788T/C polymorphism and 2 diabetes mellitus with coronary heart disease in Han population of China

目的探讨中国汉族人群载脂蛋白M（apolipoprotein M，ApoM）基因－778T／C单核苷酸多态性与2型糖尿病合并冠状动脉粥样硬化性心脏病（冠心病）的相关关系。方法采用聚合酶链反应－限制性片段长度多态性方法对238例糖尿病合并冠心病的患者及236例对照组进行检测，分析ApoM基因－778T／C多态的基因型和等位基因频率的分布情况。结果ApoM基因－788T／C多态三种基因型（TT型，TC型和CC型）在2型糖尿病合并冠心病组分布频率分别为73．9％、24．3％和1．7％，在对照组分布频率分别为86．0％、13．1％和0．9％；两组比较差异有统计学意义（P=0．001）。ApoM基因－788T／C多态T、c等位基因的频率在2型糖尿病合并冠心病组及对照组中分布分别为86．1％、13．9％和92．6％、7．4％，两组比较差异有统计学意义（P=0．001）。Logistic回归校正性别、年龄、体质量指数、吸烟、原发性高血压、高脂血症等2型糖尿病合并冠心病的易患因素后。ApoM基因-778T／C多态与2型糖尿病合并冠心病的发病仍存在相关关系（OR=2．0，95％CI：1．3～3．0）。结论ApoM基因-778T／C多态可能是中国汉族人群2型糖尿病合并冠心病患者发病的独立危险因子。

Objectives To explore the association between the apolipoprotein M （Apo M） gene -788T/C polymorphisms and type 2 diabetes mellitus with coronary heart disease in Chinese Han population. Methods A case-control study was conducted in 238 patients with type 2 diabetes mellitus complicated with coronary heart disease and 236 controls. Polymorphic genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism. Results The genotype frequencies in Apo M gene -788T/C polymorphism conformed well with the Hardy-Weinberg equilibrium in both case and control groups. The genotype frequencies of TI＇, TC and CC of Apo M gene -788T/C polymorphism were 86.0%, 13.1% and 0.9% in the controls, 73.9%, 24.3% and 1.7% in patients with type 2 diabetes mellitus with coronary heart disease respectively. Allele frequency distributions ofT, C ofApo M gene -788T/C polymorphism were 86.1%, 13.9% and 92.6%, 7.4% in case and control groups respectively. There were significant differences in the genotype and allele distribution of Apo M gene -788T/C polymorphism between cases and controls （P=0.001）. Logistic regression analysis with adjustments for other risk factors （i.e. gender, age, body mass index, smoking, hypertension, hyperlipidemia） revealed that the Apo M -788T/C allele carriers had correlation with the risk of type 2 diabetes mellitus with coronary heart disease （OR=2.0,95% CI： 1.3-3.0）. Conclusions The Apo M -788T/C allele may be considered as a genetic risk factor for type 2 diabetes mellitus with coronary heart disease in Han population of China.