低磷胁迫下辣椒幼苗过氧化物酶活性及其同工酶谱的分析

Study on POD Enzyme Activity and Isozyme Pattern Analysis of Peppers Under Low Phosphorous Stress

以4个辣椒品种"牛角椒"、"南纬金峰二号"、"茄门椒"和"8819线椒"为试材,采用水培方法,研究了常磷(1mmol/L)和低磷(0.01mmol/L)2个磷水平处理对不同辣椒品种叶片中过氧化物酶(POD)活性及其同工酶酶谱的影响。结果表明:低磷胁迫下,"牛角椒"、"茄门椒"和"8819线椒"的POD活性随着胁迫时间的延长出现先升高再下降随后又升高的趋势,"南纬金峰二号"的POD活性在整个胁迫过程一直升高;过氧化物同工酶谱的变化与酶活性变化相一致,低磷组酶活性均显著高于同期常磷组,且酶谱染色深度和谱带条数均高于同期常磷组,说明POD活性与植物的耐低磷性有关。

Taking four varieties of peppers leaves of ＇ Niujiao pepper＇,＇ Qiemen pepper＇,＇ Nanweijinfeng 2＇ and ＇ 8819 line pepper＇ as test materials, with water culture,the effect of 1 mmol/L and 0. 01 mmol/L phosphorus （P） on peroxidase （POD） activity and their isoenzymic bands were studied. The results showed that POD activity in leaves of ＇Niujiao pepper＇,＇Qiemen pepper＇ and ＇8819 line pepper＇ increased firstly, decreased secondly and then increased again with time,while the POD activity of ＇Nanweijinfeng 2＇ increased during the whole test time, and the changing trend of POD isozyme zymogram was accordance with POD activity. The enzymatic activity under low phosphorus was stronger than that of normal phosphorus treatment and the isozyme bands and expression quantity of POD were more than that of normal phosphorus treatment at the same time. The result suggested that POD was related with plant against low phosphorous press.