平菇栽培基质中污染菌的分离及其对平菇菌丝生长的影响

Separation of Contaminating Microorganism from Culture Medium of Pleurorus ostreatus and Its Effect on Hypha Growth of P.ostreatus

从衡阳市蘑菇生产厂房采集正在出菇（但出菇较差）的平菇培养基质，带回实验室经分离、纯化和保存，根据菌落、菌丝、孢子、细胞的形态进行初步鉴定，并在PDA平板上用分离纯化菌株与平菇菌丝进行了对峙接种试验，观察污染菌对平菇菌丝生长的影响。结果表明：平菇培养共分离获得8个菌株，编号分别为PoD1-PoD8，经形态初步鉴定，PoD1、PoD2和PoD3分别为黄色单胞菌属（Flavimonas）、枯草芽孢杆菌（Bacillussubtilis）和假单孢菌属（Pseudomonas），PoD4和PoD5为红酵母属（而odolorula）的2个种，PoD6为裂殖酵母属（Schizosaccharomyces），酵母菌PoD7和PoD8分别为好食脉孢菌（Neurosporasitophila）和灰绿曲霉（Aspergullusglaucus）。对峙接种结果显示，丝状真菌对平菇菌丝生长的抑制效果最明显，其次为细菌，分离的酵母菌对平菇菌丝生长的影响最小。

Culture medium of Pteurorus ostreatus were collected from mushroom production plant in Hengyang area and took back to the laboratory to deal. The strains,separated from the medium,were purified and identified by colony and microscopic morphology. Then mycelium of each one separated strains and P. ostreatus were inoculated on the same PDA flat to study the effects of isolated strains on the growth of P. ostreatus. The results showed that eight strains,numbered PoD1-PoD8 were isolated and identified by morphology, POD1, PoD2 and PoD3 were Flavimonas sp. ,Bacillus subtilis and Pseudomonas sp. respectively;PoD4 and PoD5 were Rhodolorula spp. , PoD6 was Schizosaccharomyces sp. ;POD7 and PoI）8 were Neurospora sitophila and Aspergillus glaucus respectively. The results of confrontation inoculation showed that moulds had significant inhibition effect on the growth of P. ostreatus,then the bacteria had a certain inhibition effect, the last were yeasts.