腺苷和ATP对大鼠肾脏缺血再灌注损伤保护作用的比较

COMPARISON OF PROTECTIVE EFFECT OF ADENOSINE AND ATP IN RAT RENAL DAMAGE OF ISCHEMIA-REPERFUSION

目的 :探讨腺苷和ATP对肾脏缺血再灌注损伤的保护作用机制并进行相互比较。方法 :将大鼠随机分为对照组 (CON)、缺血再灌注组 (IR)、给予ATP后缺血再灌注组 (ATP)和给予腺苷后缺血再灌注组 (AD) ,分别观察各组中在再灌注 0h、2h和 6h的Na+和K+-ATP酶和Ca2 +-ATP酶活性的变化 ,以及组织病理变化。结果 :病理学组织学肾小管评分 ,腺苷组和CON组之间无显著性差异 (P >0 .0 5 ) ,但两组均明显低于IR组和ATP组 (P <0 .0 5或P <0 .0 1) ,IR组和ATP组之间无显著差异 (P >0 .0 5 )。Na+,K+-ATP酶和Ca2 +-ATP酶的活性 ,在腺苷组和CON组之间无显著性差别 ,(P >0 .0 5 ) ,但两组均明显高于IR组和ATP组 (P <0 .0 1) ,而IR组和ATP组之间无显著性差异 (P >0 .0 5 )。结论 :腺苷对大鼠肾脏缺血再灌注损伤没有保护作用 ,ATP对大鼠肾脏缺血再灌注损伤有保护作用。

Objective: To investigate the protective machanism of adenosine and ATP in renal damage of ischemia-reperfusion. Methods: Rats were randomly divided into control group (CON), adenosine group (AD), ATP group, ischemia-reperfusion group (IR). In different groups, besides pathological changes, the activity of Na +, K +-ATPase and Ca 2+ -ATPase was measured by biochemical detection respectively. Results: The score of renal tubules in Adenosine and CON was lower than that in IR and ATP ( P <0.05 or P <0.01), and the score was similar between Adenosine and CON ( P >0.05), so was IR and ATP ( P >0.05). the activity of Na +, K +-ATPase and Ca 2+ -ATPase was no difference between Adenosine and CON ( P >0.05), but the activity of the two ATPase was higher than that in IR and ATP ( P <0.01 or P >0.05), IR and ATP was no difference ( P >0.05). Conclusions: Adenosine could protect the damage of ischemia-reperfusion, ATP couldn't protect the damage of ischemia-reperfusion.