摘要
目的:评价 MTT法测定肿瘤细胞体外药物敏感性的临床应用。方法:对75例胃癌、55例肠癌、55例膀胱癌及6例舌癌进行肿瘤细胞分离、培养,以MTT法对其体外药物敏感性进行检测。结果:对胃癌、肠癌、膀胱癌敏感度较高的药物与临床经验用药基本相符。75例胃癌病人体外药敏结果和体内疗效总符合率为77.3%,其中MTT敏感组病人总有效率与经验化疗组相比有显著性差异(P<0.01)。MTT法对新药的临床应用也有较好的指导作用。结论:MTT法对于临床合理用药,实现肿瘤病人的个体化化疗具有重要的价值。
OBJECTIVE: To evaluate the in vitro MTT assay for determining the drug sensitivity in fresh human tu- mors. METHODS: We tested the samples from 75 patients with gastric cancer, 55 patients with colorectal carcinoma, 55 patients with bladder cancer and 6 patients with carcinoma of tongue. RESULTS: On the average, the MTT sensitive drug in the above - mentioned first three kinds of solid tumors corresponded with the drug used by clinican experience. The response rate in MTT sensitive group was 73. 8% (48/65) while only 34.0% (17/50) in the control group and 0% (0/10) in MTT resistant group. CONCLUSION: MTT assay appears to be useful in exploring the possible anticancer effect of arsenic trioxide on tongue cancer. This test demonstrats that the MTT assay is clinically useful on an individual basis to optimize chemotherapy.
出处
《中国药房》
CAS
CSCD
2000年第6期254-255,共2页
China Pharmacy
关键词
MTT法
化疗
肿瘤
药物敏感性
MTT assay
chemotherapy
drug sensitivity test