有氧运动联合膳食控制对2型糖尿病大鼠Resistin分泌的影响

Effects of Aerobic Exercise and Dietary Control on Serum Resistin Concentration in Type 2 Diabetic Rats

目的:探讨有氧运动联合膳食控制对2型糖尿病(T2DM)大鼠Resistin分泌的影响。方法:雄性SD大鼠62只,随机抽取8只大鼠作为正常对照组(C),喂以标准普通饲料。其余54只SD大鼠在喂饲高糖高脂膳食的基础上腹腔注射小剂量STZ,建立T2DM动物模型。然后将T2DM大鼠随机分成4组:DM对照组(DM,n=9)、DM+运动锻炼组(DME,n=10)、DM+膳食控制组(DMD,n=10)、DM+运动锻炼+膳食控制组(DMED,n=10)。DM组大鼠继续喂饲高脂高糖饲料,不进行运动锻炼;运动锻炼采用每天进行60min的无负重游泳运动,每周6次;膳食控制采用与DM组等量的标准饲料。13周后,检测各组大鼠FPG、TG、TC、FINS、FFA和Resistin的含量。结果:①与C组相比,DM组大鼠FPG和血清Resistin含量显著升高,FINS含量显著降低(P<0.01);有氧运动可以降低T2DM大鼠FPG和血清Resistin含量(P<0.05),虽可使FINS升高,但无显著性差异;而膳食控制对降低T2DM大鼠FPG和血清Resistin含量没有显著性影响(P>0.05),但可使FINS显著升高(P<0.05);有氧运动联合膳食控制对降低T2DM大鼠FPG、血清Resistin含量和升高FINS无显著的交互作用(P>0.05)。②DM组血清TC、TG和FFA含量均显著高于C组(P<0.01);有氧运动或膳食控制均能显著地降低T2DM大鼠血TC(P<0.05)和FFA含量(P<0.01)。饮食控制能显著降低T2DM大鼠TG和FFA含量(P<0.01)。有氧运动联合饮食控制可使T2DM大鼠的TG、TC和FFA含量进一步降低,但对降低TG和FFA含量无显著的交互作用(P>0.05),对降低TC具有显著的交互作用。结论:①T2DM大鼠Resistin分泌显著增多,与糖脂代谢紊乱有关。②有氧运动能显著降低T2DM大鼠Resistin的分泌,增加胰岛β细胞分泌胰岛素的能力,对改善糖脂代谢具有非常重要的作用。而单纯的膳食控制对降低T2DM大鼠Resistin的分泌影响不显著,且有氧运动联合膳食控制对降低T2DM大鼠Resistin的分泌也无显著的交互作用。

Objective ：To explore the effect of aerobic exercise and dietary control on the serum Resistin concentration in type 2 diabetic rats. Method： 8 rats were randomly selected from 62 male SD rats as normal control group （ C）, which fed with normal standard diet. The remaining 54 rats which were injected low-dose streptozotocin though abdominal cavity to establish T2DM animal models based on feeding high-fat and high-carbohydrate diet. Then the T2DM rats were randomly divided into 4 groups ： DM control group （ DM, n = 9 ）, DM ＋ aerobic exercise training group （ DME, n = 10 ）, DM ＋ Diet control group（ DMD, n = 10）, DM ＋ aerobic exercise training ＋ Diet control group （DMED, n = 10 ）. The rats DM group were contimually fed with high-fat and high-carbohydrate diet and had no exercise. Exercise were used 60 minutes＇ unloa- ded swimming for 13 weeks, 6 times per week. The dietary control use equivalent standard diet with the DM group. 13 weeks later,FPG,TG,TC ,FINS and Resistin in each group were examined. Results： （.~）Compared with group C, the FPG and serum Resistin contents increased significantly, serum insulin concentration decreased significantly in DM group （P 〈 0.01 ） ;aerobic exercise could lower FPG and serum Resistin contents（P 〈 0.05 ）, and it could increase FINS level in dia- betic rats, but there was no significant difference （ P 〉 0.05 ） ; And dietary control had not significantly effect on FPG and serum Resistin in diabetic rats （ P 〉 0.05 ）, but it could increase FINS significantly （ P 〈 0.05 ）. Exercise joint dietary con- trol could further decrease FPG and serum Resistin and increased FINS in T2DM rats ,but there were not obvious interac- tion （ P 〉 0.05 ）. （2）Compared with C group, the serum TC, TG and FFA concentration increased significantly （ P 〈 0.01 ） ; both of aerobic exercise and dietary control could significantly lower serum TC and FFA concentration （ P 〈 0.05, P 〈 0. 01 ） in T2DM rats. Dietary control could lower TG and FFA concentration in type 2 diabetic rats（ P 〈 0.01 ） significantly. Aerobic exercise joint dietary control could further lower serum TG,TC and FFA concentration in T2DM rats ,but it had no significant interaction on decreasing TG and FFA（P 〉 0.05 ）, however had a significant interaction on decreasing TC. Con- clusions：（1）The Resistin secretion in T2DM rats significantly increased, which had a correlations with disorders of glucose and lipid metabolism. @Aerobic exercise could significantly reduce Resistin secretion in T2DM rat, increase β-cell secre- tion of insulin＇s ability, which had a very important role on improving glucose and lipid metabolism. A simple diet control had no significant effect on reducing the Resistin secretion in T2DM rat, and aerobic exercise combined dietary control had no significant interaction on reducing the Resistin secretion in T2DM rat also.