甘肃不同产地中麻黄遗传关系的ISSR分析

Genetic Relationship Analysis of Ephedra intermedia from Different Habitat in Gansu by ISSR Analysis

目的:研究甘肃不同产地中麻黄间的遗传关系。方法:应用ISSR分子标记技术对甘肃10个不同产地的中麻黄样品进行遗传多样性和亲缘关系分析。结果:从70个ISSR引物中筛选出12个条带清晰、多态性高的引物,共检测出112位点,其中多态性位点81个,多态性条带比率(PPB)为72.32%。聚类结果显示来自不同地区的野生种和栽培种被分开各自聚成一类,地理距离较近的野生种聚为一类。结论:甘肃不同产地中麻黄在物种水平上具有丰富的遗传多样性,中麻黄的遗传距离与地理距离具有一定的相关性。

Objective： To investigate the genetic relationship of Ephedra intermedia from different habitats in Gansu. Methods： The genetic diversity and genetic relationship of E. intermedia from different habitats in Gansu were studied by ISSR molecular marker technique. Results： Twelve ISSR primers were selected from 70 ISSR primers and used for ISSR amplification. Total 112 loci were amplified, in which 81 were polymorphic loci, the average percentage of polymorphie bands（PPB） was 72. 32%. Clustering results indicated that the wild species and cultivating species were clustered into different group. The wild species, which had closer distance, were clustered into a group, Conclusion ： E. intermedia of diflbrent habitats in Gansu have rich genetic diversities among species, it is the reason that E. intermedia has strong adaptability and wide distribution. Further,the genetic distance of E. intermedia is associated with geo-graphical distance,the further distance can hinder the gene flow.