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分光光度法测定新疆不同产地白喉乌头不同部位总生物碱的含量 被引量:1

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摘要 目的:测定新疆不同产地白喉鸟头不同部位总生物碱的含量。方法:采用分光光度法,以乌头碱为对照品,溴甲酚绿为酸陆染料,缓冲溶液pH3条件下,三氯甲烷萃取45min,于415nm波长处测定其吸光度。结果:乌头碱在1.26~16.80mg/L浓度范围内线性关系良好,回归方程Y=22.506x-2.3916,r=0.9998;平均加样回收率为100.06%,RSD为1.66%。结论:新疆白喉乌头总生物碱的含量较高,其中以根部含量最高,茎叶和花果实中总生物碱含量略低,但电均高于已有法定药材标准的草乌中总生物碱的含量,值得进一步合理开发利用。
出处 《中药材》 CAS CSCD 北大核心 2013年第9期1406-1408,共3页 Journal of Chinese Medicinal Materials
基金 国家自然科学基金(81160498)
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