何首乌生品与炮制品对大鼠肝脏的毒理学研究

Study on the Hepatoxicity of Polygoni Multiflori Radix and Polygoni Multiflori Rodix Praeparata in Rats

目的:比较中药何首乌生品与炮制品对大鼠的肝毒性。方法:常规煎煮获取生何首乌与制何首乌水提物(分别命名为RPWE和PPWE);分别用RPWE和PPWE灌胃SD大鼠,均按何首乌生药量设低剂量组[20 g/(kg·d)]和高剂量组[40 g/(kg·d)],分别进行30 d及60 d实验;观察用药后大鼠的一般状况,并于实验结束时采集大鼠血液进行丙氨酸氨基转移酶(ALT)与天门冬酸氨基转移酶(AST)检测,同时测定大鼠肝重,计算肝脏系数,对大鼠肝脏进行常规组织切片和HE染色。结果:30 d及60 d实验中,给药组大鼠较空白对照组大鼠表现怠动、毛色不华且有腹泻现象,随着药物浓度的增大,怠动、毛色不华以及腹泻现象加重;灌胃60 d,给药组大鼠体重较空白组大鼠明显变轻,且给药浓度愈大,大鼠体重愈轻;30 d及60 d实验中,给药组大鼠肝脏系数均没有明显变化。大鼠血液生化检测发现,各给药组相对于空白对照组转氨酶(ALT、AST)没有显著性增加,但是灌胃60 d的大鼠肝脏切片HE染色发现均有不同程度的脂肪变性,炎性细胞浸润等。结论:生、制何首乌灌胃60 d,肝脏有轻度脂变及炎性细胞浸润。

Objective ：To compare the hepatoxicity of Polygoni Muhiflori Radix and Polygoni Muhiflori Rodix Praeparata in rats. Methods ： Water extracts of Polygoni Multiflori Radix and Polygoni Muhiflori Rodix Praeparata were prepared by coneentional deeocting method （named as RPWE and PPWE respectively）. Both in 30 days and 60 days experiments, SD rats were randomly divided into 5 groups：normal control group, RPWE high dosage group （40 mg/kg · d） and PPWE low dosage group （20mg/kg · d）, PPWE high dosage group （40 mg/kg · d） and PPEW low dosage group（ 20 mg/kg · d）. After daily administration, the general conditions of rats were observed. At the end of the experiments, the levels of ALT and AST in plasma were detected. The liver coefficient was calculated and HE staining was used to observe the changes of liver tissue sections. Results：Both in 30 days and 60 days experiments, the treatment groups rats had hypokinesia and dry fur when compared with control groups, and in a dose-dependent manner. The liver coefficients, levels of ALT and AST of plasma weren＇t changed significantly when conpared with control groups. The liver had varying degrees of steatosis and inflammation cell infiltration in the treatment groups rats in 60 days experiment. Conclusion ： Oral administration of Polygoni Multiflori Radix and Polygoni Muhiflori Rodix Praeparata for 60 days have hepatoxic in rats.