芦荟大黄素对高转移乳腺癌细胞MDA-MB-231侵袭与转移的影响

Effect of Aloe Emodin on Invasion and Metastasis of High Metastatic Breast Cancer MDA-MB-231 Cells

目的:研究芦荟大黄素(Aloe emodin,AE)对人高转移乳腺癌细胞MDA-MB-231侵袭与转移的影响。方法:MTT法检测AE对MDA-MB-231细胞增殖的抑制作用;细胞-基质粘附试验检测AE对MDA-MB-231细胞粘附纤连蛋白(FN)、层连蛋白(LN)能力的影响;Transwell chamber法检测AE对MDA-MB-231细胞侵袭重组基底膜能力的影响;伤口愈合试验检测AE对MDA-MB-231细胞迁移能力的影响。裸小鼠模型观察AE对MDA-MB-231细胞实验性转移的影响。结果:80μmol/L AE能显著抑制MDA-MB-231细胞体外侵袭重组基底膜能力,显著抑制MDA-MB-231细胞粘附FN、LN的能力,其抑制率分别为(52.98±5.46)%,(34.99±2.63)%,(28.73±7.00)%。作用于MDA-MB-231细胞24 h后,AE能显著抑制MDA-MB-231细胞的迁移,80μmol/L AE处理的MDA-MB-231细胞在裸小鼠中形成肺转移结节的数量、体积明显减少。结论:AE抑制MDA-MB-231细胞的转移,其作用机制与抑制MDA-MB-231细胞的侵袭和迁移能力有关。

Objective：To investigate the effect of Aloe emodin （AE）on the invasive and metastatic abilities of human high meta- static breast cancer MDA-MB-231 ceils. Methods：MTF assay was used to evaluate the viability of MDA-MB-231 ceils after treated with AE for 6 h and 24 h. The adhesive potential of MDA-MB-231 cells to FN and LN was tested by cell-matrix adhesion assay. The effect of AE on invasion of MDA-MB-231 cells was measured by Transwell chamber assay. Scratch wound healing assay was applied to determine the effect on migration of MDA-MB-231 ceils. The effect of AE on MDA-MB-231 lung metastasis was determined on an experimental metastatic model. Results： 80 μmol/L AE significantly inhibited the invasion, adhesion to FN, LN of MDA-MB-231 ceils in vitro, the inhibitory rates were （ 52. 98 ± 5.46 ） %, （ 34. 99 ± 2. 63 ） %, （ 28.73 ± 7.00 ） %, respectively. After 24 h treatment, AE significantly inhibited the migration of MDA-MB-231 cells. The number and volume of lung metastatic nodules formed by MDA-MB-231 cells after 80 μmol/L AE 24 h treatment were decreased compared with control group. Conclusion：AE can suppress the metastasis of MDA-MB-231 cells. Their mechanisms may be related to the inhibition of the capabilities of invasion and migration of MDA-MB-231 cells.