中药提取物(-)-Antofine脂质体的制备及抗乳腺癌活性研究

Preparation and Anti-breast Cancer Activity of(-)-Antofine Carried with Liposomes

目的:制备活性高、水溶性好的(-)-Antofine脂质体,并进行其体内外抗乳腺癌活性研究。方法:采用薄膜分散法制备(-)-Antofine脂质体,用紫外分光光度仪测定(-)-Antofine脂质体包封率,用MTT法测定(-)-Antofine脂质体细胞活力,通过荷瘤小鼠实验评价体内抑瘤活性和主要脏器指数。结果:用薄膜分散法成功制得了(-)-Antofine脂质体,平均包封率为80.8%±2.2%,平均载药量为26.7%±1.4%,(-)-Antofine脂质体具有较好的水溶性,体内外实验结果均表明(-)-Antofine脂质体的抗乳腺癌活性较(-)-Antofine原药有所提高。结论:(-)-Antofine脂质体具有较高的包封率,提高了(-)-Antofine水溶性和抗肿瘤活性。

Objective： To prepare （ - ）-Antofine liposomes with high activity and good water-soluble and study its anti-breast cancer activity both in vitro and in vivo. Methods： （ - ）-Antofine liposomes were prepared by film dispersion method, the encapsulation efficiency of （ - ）-Antofine liposomes were determined by UV spectrophotometer,the cytotoxic activity of （ - ）-Antofine liposomes on MCF-7 cell was measured by MTY assay, the antitumor activity and major organs indexes were evaluated by tumor-bearing nude mice experimental in vivo. Results ： The average encapsulation efficiency of （ - ） -Antofine liposomes was （ 80. 8 ± 2. 2 ） % , average drug loading was （26. 7 ± 1.4 ） %, it was relatively good water-soluble and had good anti-tumor activity in vitro and in vivo. Conclusion ： （ - ）-Antofine liposomes prepared by film dispersion method has high encapsulation efficiency, the water-soluble and the anti-tumor activity are improved compared with （ - ）-Antofine.