酶法测定血清肉毒碱方法学研究

Enzyme Reagents Method for the Determination of Carnitines in Serum

目的 :建立简便 ,快速测定血清总肉毒碱和游离肉毒碱的方法。方法 :用自动分析仪在 42 0nm测定肉毒碱乙酰转移酶催化L 肉毒碱与乙酰CoA反应中CoA的生成量。结果 :①方法的线性为 0～ 2 0 0 μmol L ,天内和天间变异糸数分别为 3 .4%和 4.6 % ,平均回收市率 99.8% ,乳糜血、黄疸、溶血标本对测定结果均无影响。②本法的参考值 ( x±s) :总肉毒碱和游离肉毒碱分别为 (5 8.2± 8.0 ) μmol L和 (49.2± 7.6 ) μmol L ,42例肝硬化标本为 (92 .8± 11.9) μmol L和 (70 .5±11.5 ) μmol L ,两者均数比较差异显著 (P <0 .0 1)。结论 :用本法测定血清总肉毒碱和游离肉毒碱 ,简便可靠 ,可为肝硬化的特异诊断提供进一步的参考数据。

Objective: To establish a simple and raped method for serum total carnitine and incarnitine determination. Methods: Use automation analysis apparatus on 420nm wave length for the determination of the production of CoA to catalyse the reaction of carnitineis and acetyl CoA. Results: ①The linear range of the method was 0～200μmol/L.The CVs within the day and spacing day were 3.4% and 4.6% respectively.The average recovery was 99.8%.The measurement of carnitines was not affected by lipidemia, icterus and solvent blood specimen. ②clinical reference value of this method ( ±s ):Total carnitines and ioncarnitine were (58.2±8.0)μmol/L and (49.2±7.6)μmol/L respectively. But in 42 hepatocirrhosis blood specimen they were (92.8±11. 9)μmol/L and (70.5± 11. 5) μmol/L respectively. There were significant statistical differences between the two groups( P < 0. 01) . Conclusion: Determination of total carnitines and incarnitines with this method was simple and quasi stationary and provides further information for the differential diagnosis of hepatocirrhosis.