4个不同居群新疆阿魏中挥发性成分分析

Analysis on Chemical Constituents of the Volatile Oil from Four Different Populations of Ferula sinkiangensis K. M. Shen

目的通过对4个不同居群新疆阿魏树脂挥发性化学成分的比较研究,为新疆阿魏遗传多样性的研究提供科学依据。方法通过水蒸汽蒸馏法提取新疆阿魏树脂挥发油,采用GC-MS技术分析、鉴定其挥发性成分,以GC-MS分析的主要化学成分及其相对含量为检测指标,对野生生长在同一生境下的4个不同居群新疆阿魏树脂成分进行了比较分析。结果从新疆阿魏各居群挥发油中分出了27个色谱峰,鉴定了22个化合物,4个不同居群新疆阿魏挥发油GC-MS分析均含有相同的主要活性成分,其相对含量差异均没有统计学意义,其中3,4-二甲基噻吩、α-萜品烯、水芹烯、3-蒈烯、2,3,4-三甲基噻吩、3,6-二甲基-1,4-二噻烷-2,5-二酮、3-硫代丙酸甲酯、1,1-二甲硫基乙烷、1,1-二甲氧基丙烷、α-石竹烯和绿叶烯为首次在新疆阿魏药材中检测到。结论综合分析新疆阿魏药材挥发性成分化学信息,新疆阿魏4个居群GC-MS分析主要化学成分一致,其相对含量差异均没有统计学意义,为新疆阿魏种质资源、道地性与品质评价的重要依据。

Objective In order to study the genetic diversity of Ferula sinkiangensis K. M. Shen., we carried out comparative analysis on chemical constituents of the volatile oil from four different populations of Ferula sinkiangensis K. M. Shen. Methods The chemical constituents of the volatile oil were extracted from four different populations of Ferula sinkiangensis by applying steam distillation, and then were analyzed and identified by using GC-MS method. The mainly chemical constituents of the volatile oil and theirs relatively contents as markers, four populations of Ferula sinkiangensis, which wildly grows in the same environmental conditions, was comparatively analysed. Results There were totally 27 constituents of the volatile oil extracted and identified from four different populations ofFerula sinkiangensis. The contents of the volatile oil were all more than 10 percent in different populations of Ferula sinkiangensis. And the mainly chemical constituents of the volatile oil and theirsrelatively contents were almost same. Conclusion With the all aspects information on chemical constituents of the volatile oil from Ferula sinkiangensis, Ferula sinkiangensis K. M. Shen, which only grows in Baishitu country of Xinjiang region of China, had an important influence on the quality evaluation and authentic and superior Chinese medicinal materials of Resin Ferula sinkiangensis.