微生物发酵制备莽草酸的含量测定

Determination of Shikimic Acid in the Production of Microbial Fermentation

由于微生物发酵法合成莽草酸的研究尚处于实验室研究阶段,建立了微生物发酵制备莽草酸含量的测定方法。采用紫外可见分光光度法对莽草酸产生菌发酵液中莽草酸进行测定。检测波长245 nm,莽草酸标准溶液线性方程为:A=3.084 4C-0.004 8,R2=0.999 9。平均回收率为100.04%,RSD为0.32%。方法简便、快速、准确,且稳定性好,适用于测定微生物发酵生产莽草酸的含量。

The method for determination of shikimic acid in the production of microbial fermentation was established.Using ultraviolet-visible spectrophotometry for determination of shikimic acid in the fermented liquid of shikimic acid producing bacteria.The detection wavelength was 245 nm,Linear equation of shikimic acid standard solution was A =3.084 4C-0.004 8 （R2=0.999 9）.The recovery was 96.7%～103.7% with average recovery of 100.04%,RSD was 0.32%.The method is simple,fast,stable and accurate,and suitable to determine the shikimic acid in the production of microbial fermentation.