吉林省大豆骨干亲本及主推品种DNA指纹图谱的构建

DNA fingerprint of the main parents and current varieties of soybean in Jilin province

以 64份吉林省大豆骨干亲本及主推品种为试材 ,利用 RAPD分子标记技术 ,经过 1 4 0个引物的重复筛选 ,构建了一套完整的 DNA指纹图谱 ,其中 OPA-0 4能区分 4 0份材料 ,结合引物 OPG-0 7、OPO-1 1、OPF-1 4的扩增结果即可完全区分供试 64份材料。聚类分析表明 ,具有相同骨干亲本的育成品种遗传距离较近 ,这与以往的农艺性状研究及生理生化研究结论相符。

Sixty four main parents and varieties of soybean in Jilin province were studied in this experiment. By utilizing RAPD (Random Amplified Polymorphism DNA), a DNA fingerprints was constructed by screening with 140 primers. Only primer OPA 04 could distinguish 40 of all the 60 varieties from each other and all the materials could be distinguished with primer OPG 07, OPO 11 and OPF 14. Dendrogram analysis showed genetic relationships among those varieties which have the same genetic resources. Therefore, the results showed that the RAPD markers can be used for identification of seed purity and varieties, which can be used in soybean breeding and production and also be very essential to varieties protection and intellectual property right.