摘要
阿特拉津的大量使用在全球范围内受到广泛的关注,并且进入各国的优先污染物监测范围。本文建立了超高效液相色谱-串联质谱(UPLC-MS/MS)检测阿特拉津的方法。水样经过简单的0.2μm滤膜过滤之后即可进样,实际样品加标的方法回收率达到108.5-115.1%。采用Acquity BEH C18色谱柱,以甲醇和水为流动相,以甲酸作为缓冲溶液,辅以电喷雾正离子模式多反应监测(MRM)进行定性定量分析。方法检测限达到0.03μg/L,与之前的研究相比明显提高了灵敏度。与目前国家标准方法相比较,本方法通过质谱定性的方式避免了紫外或荧光检测器可能出现的误判现象;在定量方面,本方法极大地提高了灵敏度;同时,实现了地表水等实际环境样品的前处理过程的极大简化,减少了萃取溶剂,节约了时间和成本,适宜用于水样中阿特拉津的检测。
As the most widely used pesticides,atrazine has attracted extensively focus and is on the priority list released by many countries.A UPLC-MS/MS method is developed for the determination of atrazine in water samples.Water samples could be injected into instruments only after filtering the solution with 0.2 μm membrane.The percent recovery of the spike ranged from 108.5-115.1%.The method included an Acquity BEH C18 column separation operated with a gradient elution solvent of methanol and ultrapure water coupled to a mass Spectrometry scanned in multiple reaction monitoring （MRM） mode and isotope dilution.The limit of detection （LOD） of the method reached 0.03 μg/L,lower than the previous research. Compared with the method of China national standards for drinking water quality, this novel method has great improvement both in qualitation and quantification.Furthermore,the simplification of the sample preparation process makes the analysis of atrazine quickness,simplicity, and economy.It meets the requirement of routine screening of atrazine residues in water samples.
出处
《现代科学仪器》
2013年第5期120-123,共4页
Modern Scientific Instruments
