液相色谱法测定饮料中合成着色剂的不确定度评定

Evaluation of Uncertainty in Determination of Synthetic Colour in Drink by High Performance Liquid Chromatography

目的:评估液相色谱测定法[1]对饮料中合成着色剂检测结果的影响。方法:根据测定方法建立数学模型,然后对不确定度的分量逐一进行计算,最终合成不确定度报告。结果:当k=2(95%置信概率)时:柠檬黄的含量为(12.1±0.6)μg/kg,苋菜红的含量为(9.96±0.48)μg/kg,靛蓝的含量为(10.0±0.5)μg/kg,胭脂红的含量为(9.98±0.46)μg/kg,日落黄的含量为(9.98±0.48)μg/kg,诱惑红的含量为(10.0±0.5)μg/kg,亮蓝的含量为(10.0±0.5)μg/kg,赤藓红的含量为(10.0±0.7)μg/kg。结论:评估对液相色谱法测定饮料中合成着色剂过程的质量控制及检测结果可信度的保证有实际意义。

Objective：The effect on determination of synthetic colour in drink is evaluated by high performance liquid chromatography.Method：According to the measurement method,the mathematic model was established,then the ponderance of uncertainty was calculated and the report of uncertainty was gotten.Results：When k=2,1emon yellow in drink was （12.1±0.6）μg/kg and amaranth in drink was （9.96±0.48）μg/kg and Indigo in drink was （10.0±0.5）μg/ kg and ponceau 4R in drink was （9.98±0.46）μg/kg and sunset yellow in drink was（9.98±0.48）μg/kg and allura red in drink was （10.0±0.5）μg/kg and brilliant blue in drink was （10.0±0.5）μg/kg and erythrosine in drink was （10.0±0.7）μg/ kg.Conclusion：This method of evaluation synthetic colour in drink can be used in quality control of determination process