摘要
利用Agilent Rat全基因组芯片技术和实时荧光定量PCR技术,采用60Coγ射线对SD大鼠全身照射,照射剂量为2.0 Gy,分析照后6、12、24小时大鼠外周血淋巴细胞的差异表达基因谱和通路,同时对基因芯片结果进行验证。结果表明:(1)照后6小时,差异表达基因有1 084个,其中上调的736个,下调的348个;照后12小时,差异表达基因有2 590个,其中上调的1 621个,下调的969个;照后24小时,差异表达基因有3 938个,其中上调的2 278个,下调的1 660个;3个时间点共同差异表达基因有446个,其中上调的274个,下调的172个。(2)照后6小时,差异表达基因涉及到的通路有18个;照后12小时,差异表达基因涉及到的通路有35个;照后24小时,差异表达基因涉及到的通路有38个。其中通路Cell adhesion molecules、ΦToxoplasmosis、ΦB cell receptor signaling、ΦIntestinal immune network for IgA Production等在照后3个时间点均有出现。(3)差异表达基因Trmt61a和Enc1的相对定量结果与基因芯片检验结果表达趋势一致。
The study analyzed the differential expression profile and pathway of peripheral blood lymphocyte in SD rats 6 ,12 and 24 hours after irradiation to 2 .0 Gy gamma ray .The results showed that at 6 hours after ir-radiation there were 1084 differentially expressed genes of which 736 genes were up-regulated and 348 genes down-regulated .12 hours after irradiation there were 2590 differentially expressed genes of which 1621 genes were up-regulated and 969 genes down-regulated .24 hours after irradiation there were 3938 differentially ex-pressed genes of which 2278 genes were up-regulated and 1660 genes down-regulated .There were 446 co-ex-pressed differential genes at three time points ,of which 274 genes were up-regulated and 172 genes down-regu-lated .The KEGG analysis revealed that 6h point 18 pathways ,12 hours point 35 pathways ,and 24 hours point 38 pathways involved .RT-PCR results indicated that the relative quantity’s results of Trmt61a and Enc1 were consistent with gene chip data .
出处
《辐射防护通讯》
2013年第4期1-7,共7页
Radiation Protection Bulletin
关键词
大鼠
淋巴细胞
基因表达
基因芯片
Γ射线
Rat
Lymphocyte
Gene chip
Differential expression gene
Gamma-ray