摘要
目的:体外观察60味中药的240种提取物对9种常见病原菌的作用,并对其中抑菌效果明显的提取物进行最低抑菌浓度检测。方法:采用纸片扩散法对240种提取物进行初步的抗菌活性筛选,并采用液体培养基法(2倍稀释法)测定活性提取物的最低抑菌浓度(MIC)。结果:共有104种提取物对1种或多种细菌显示较强的抑制作用,其中丹参等11味中药的20种提取物对3种常见菌体现了强抑制作用(MIC<0.2 mg·mL-1)。结论:丹参、蛇床子、蓼蓝、蒲公英、桑叶、甘草、姜黄、紫草、半边莲、野菊花和密蒙花等11味中药的乙醇、丙酮、正己烷提取部位对枯草芽孢杆菌、蜡样芽孢杆菌和金黄色葡萄球菌有强抑制作用。
This study was aimed to screen antibacterial agents against 9 pathogenic bacteria from 240 extracts of 60 traditional Chinese medicinals （ TCM ） . And the minimum inhibitory concentration （ MIC ） test was applied on extracts with positive results. The disk diffusion was employed to screen the antibacterial activity preliminar-ily among 240 extracts. The MICs of active extracts were tested by liquid culture method （double dilution method）. The results revealed that 104 extracts show antibacterial activity on one or more strains, 20 of them show strong inhibition on three commonly seen bacteria （ MIC 〈 0 . 2 mg/mL ） . It was concluded that ethanol , acetone and hexane extracts of 11 TCMs including Salvia miltiorrhiza, Cnidium monnieri, Polygonum tinctorium, Taraxacum mongolicum, Morus alba, Glycyrrhiza uralensis, Curcuma longa, Arnebia euchroma, Lobelia chinen-sis , Chrysanthemum indicum and Buddleja officinalis show strong inhibition on Bacillus subtilis , Bacillus cereus , Staphylococcus aureus .
出处
《世界科学技术-中医药现代化》
北大核心
2013年第6期1268-1273,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
清华大学-鲁汶大学中国-比利时国际合作课题(BIL10/20T/BOF-BWS):Purification and identification of components with antifungal activity from traditional Chinese medicinal preparations,负责人:罗国安
赣南医学院校级重点课题(ZD201101):高良姜抗真菌活性成分的研究,负责人:黄浩
赣南医学院校级重点课题(PY201203):虎耳草抗金黄色葡萄球菌及蜡样芽孢杆菌活性成分及其作用机理研究,负责人:黄浩
