合欢花总黄酮对抑郁模型大鼠学习记忆能力及血浆单胺类神经递质的影响

Effects of Albizia Julibrissin Flower Total Flavonoids on Learning and Memory Abilities and Plasma Monoamine Neurotransmitters of Depression Model Rats

目的:观察合欢花总黄酮对抑郁模型大鼠学习记忆能力及血浆单胺类神经递质5-羟色胺(5-HT)、去甲肾上腺素(NE)含量的影响。方法:将90只SD大鼠随机分为正常组、模型组、西药组(盐酸文拉法辛0.012 5 g·kg-1)、合欢花总黄酮高、中、低剂量组(相当于生药量10、5、2.5 g·kg-1)。以孤养加慢性不可预见性应激建立抑郁症大鼠模型。采用Morris水迷宫评价各组大鼠的学习记忆能力,并用酶联免疫吸附实验(ELISA法)测定各组大鼠血浆中5-HT、NE的含量。结果:合欢花总黄酮大、中、小剂量连续给药21天能够明显缩短Morris水迷宫定位导航潜伏期(P<0.05或P<0.01),增加空间探索中穿越平台次数(P<0.05或P<0.01),且存在一定的量效关系;合欢花总黄酮大、中、小剂量均能提高抑郁模型大鼠血浆中5-HT、NE的含量(P<0.05)。结论:合欢花总黄酮能够改善抑郁模型大鼠的学习记忆能力,具有潜在的抗抑郁作用,其作用机制可能与提高血浆单胺类神经递质5-HT、NE含量有关。

This study was aimed to observe effects of Albizia Julibrissin Flower Total Flavonoids （AJFTF） on the learning and memory abilities and plasma monoamine neurotransmitters 5-HT and NE content of depression model rats . A total of 90 SD rats were randomly divided into the normal group , model group , western medicine group （ Venlafaxine Hydrochloride 0 . 0125 g？kg-1 ） and high , middle and low dosage groups of AJFTF （ equivalent to the crude drug of 10 , 5 , 2 . 5 g？kg-1 ） . Depression model rats were induced by solitary cultiva-tion and chronic unpredictable stress . The learning and memory abilities of model rats were evaluated with the Morris water maze . And the plasma contents of 5-HT and NE were determined by enzyme-linked immunosor-bent assay （ ELISA ） . The results showed that AJFTF （ administered with high , middle and low dosage for 21 days ） significantly shortened the incubation period of the Morris water maze positioning and navigation （ P 〈0 . 05 or P 〈 0 . 01 ） , and increased the times of crossing the platform in space exploration （ P 〈 0 . 05 or P 〈0 . 01 ） . There was a certain dose-effect relationship . The AJFTF （ high , middle and low dosage ） increased the plasma contents of 5-HT and NE （ P 〈 0 . 05 ） . It was concluded that AJFTF can improve learning and memory abilities of depression model rats which indicates that AJFTF have potential antidepressant effects . Its action mechanism may be related to increase plasma monoamine neurotransmitter content of 5-HT and NE .