双价抗菌肽基因转化辣椒

Transference of Double Gene Cecropin B and D into Pepper (Capsicum annuum L. )

在建立高效快速辣椒（Ｃａｐｓｉｃｕｍ ａｎｎｕｕｍ Ｌ，）子叶离体培养和植株再生体系的基础上，将昆虫抗菌肽Ｂ、Ｄ基因构建而成的双价质粒ｐＣＤＢ－ＩＩ以农杆菌为介导转入５个辣椒栽培品种，共获得ＫａｎＲ植株１２００多株，对部分ＫａｎＲ植株进行点杂交、ＰＣＲ、Ｓｏｕｔｈｅｒｎ杂交检测，结果证明了外源基因被成功整合。盆栽接青枯菌试验，结果显示，转基因植株具有较强的抗病力。

Based on in vitro culture system of high capacity and fast regeneration of pep- per cotyledon (Capsicum annuum L.), 5 pepper cultivars were transformed with plasmid pCDB--II constructed by double Cecropin B and D genes through mediation of A grobaterium tumefaciens. More than 1200 regenerated plants resistant to Kanamycin were obtained and grown in the greenhouse. Dot blotting, PCR detection and Southern blotting proved some of them were successfully integrated into pepper genome. The transgenic plants inoculated with pathogen of bacterial wilt (Pseudomonas solanacearum) expressed stronger resistance to bacterial wilt disease.