BA-Dot-ELISA检测牛结核乳清抗体的研究

STUDY ON DETECTING ANTIBODY OF TUBERCULOSIS IN COW MILK WHEY BY THE METHOD OF BA-Dot-ELISA

本研究建立了快速检测牛乳中结核病抗体的BA-Dot-ELISA方法。用该法检测28份结核变反阳性牛乳清，阳性率为78.57%，比常规ELISA(64.28%)高。用该法检测布鲁氏菌病牛乳清无交叉反应，但对5份副结核变反阳性牛乳清出现了一定的交叉反应。

The study was conducted by establishing the method of detecting antibody of tuberculosis in cow milk when by BA-Dot-ELISA. The results showed as follows: By using the way of BA-Dot-ELISA to detect 28 samples of milk when by PPD positively detected, the rate of positive reaction was 78.57%,it was higher than that of routine ELISA test. There was no intersect reaction by using the way to detect Brucellosis, but there was a certain intersect reaction in detecting paratuberculosis in 5 sample milk.