摘要
从自然界中分离出的黑曲霉9813株经诱变处理,获得一株高产纤维素酶的9813X8突变株。对9813X8株固态发酵产纤维素酶、半纤维素酶及果胶酶的优化条件进行了研究。通过综合评分的决策方法分析表明,黑曲霉9813X8株的产酶优化条件为:稻草与麸皮的比例1:1,料水比1:2,氮源是1.0% 硫酸铵和1.0% 豆饼,培养基起始pH值3.2~3.5,250 ml三角瓶中装培养基5~10 g,培养时间48~60 h;用0.2 mol/L、pH4.8的醋酸缓冲液室温下浸提3 h,所得粗酶液活性最高。
A mutant strain 9813X8 with high yield of Cellulase was obtained from Aspergillus Niger 9813 which was isolated from nature by treatment of Ultraviolet radiation and N-methyl-N-nitro-N-nitrosoguanidine. Using decision method of the synthetic grade, the results showed that the optimum condition of producing Cellulase, Hemlcellulase and Pectinase were as follows: the proportion of straw powder to wheat bran was 1:1,the water amount of medium was 200%, pH was at 3.2~3.5,the temperature was 30℃ and the time was 60 min, additional nitrogen compounds were 1.0% (NH4)2SO4 and 1.0% bean cake, ventilatory capacity was 5~10 g in 250 ml triangle bottle,in the solid-state medium of straw powder and wheat bran. Enzyme solution was extracted by 0.02 mol/L、pH4.8 acetum at the room temperature for 3 h.
出处
《黑龙江八一农垦大学学报》
2000年第4期61-65,共5页
journal of heilongjiang bayi agricultural university
关键词
黑曲霉
固态发酵
选育
优化条件
纤维素酶
:Aspergillus Niger
screening
solid state fermentation
optimum condition