高效液相色谱测定雪糕中色素

High performance liquid chromatography for pigments determination in ice cream

目的建立雪糕中8种人工合成色素(柠檬黄、日落黄、胭脂红、苋菜红、诱惑红、赤藓红、靛蓝、亮蓝)在可见光区变换波长的高效液相色谱测定方法。方法样品经正己烷去除油脂,再用乙醇-氨水提取,提取液挥干后用水定容,然后过滤膜后进高效液相色谱(HPLC)分析,波长选择各色素在可见光区最大吸收波长。结果 8种人工合成色素在1.0 mg/L^50 mg/L范围内线性良好,相关系数(r)都在0.999以上,方法加标回收率为90.8%~99.1%,相对标准偏差RSD(%)在1.2%~4.0%。结论该方法步骤简单,操作方便,结果精密度良好,准确度可靠。

Objective To develop high-performance liquid chromatographic method with transform wavelength in the visible region of ice cream to detect the 8 kinds of synthetic pigments（tartrazine,sunset yellow,ponceau 4R,amaranth,allura red,erythrosine,indigotine,brilliant blue）.Methods Samples was defatted with hexane,then extracted with ethanol-ammonia water,evaporated to dryness for constant volume,then filtered and analyzed with high performance liquid chromatography（HPLC）,the wavelength selection of each pigment was based on the maximum absorption wavelength in the visible region.Results Eight kinds of synthetic pigments exhibited a good linearity in the range of 1.0 mg / L ~ 50 mg / L with correlation coefficient（r） above 0.999,the recovery of the method was from 90.8% to 99.1%,the relative standard deviation RSD（%） was from 1.2% to 4.0%.Conclusion The method is simple,easy to operate,precise,accuracy and reliable.