丽水市禽流感病毒H7N9及外环境标本检测分析

Detection and analysis of human avian influenza(H7N9) virus in Lishui

目的对丽水市人感染H7N9禽流感监测病例、外环境监测标本H7N9和H5、H7、H9亚型禽流感病毒进行检测,了解禽流感病毒的分布情况。方法荧光定量PCR方法检测甲型通用流感病毒和禽流感病毒H7N9、H5、H7、H9亚型核酸。结果 4份外环境家禽粪便标本禽流感病毒H9亚型核酸检测阳性,其它标本H7N9、H5、H7、H9亚型核酸检测均为阴性。结论在本市外环境中有禽流感病毒H9亚型存在和扩散危险,目前在人群和禽类中尚未检出H7N9禽流感病毒。

Objective To understand the distribution of avian influenza virus in Lishui,and the subtypes of avian influenza virus,such as H7N9,H5,H7,H9,were detected in people and external enviromental monitoring samples.Methods Influenza A virus and avian influenza virus H7N9,H5,H7,H9 subtypes were detected by fluorescent quantitative PCR.Results Avian influenza H9 virus subtype was detected from 4 poultry feces specimens from external encironment,and other samples were all negative.Conclusion The avian influenza H9 virus subtype was found in external environment,indicating spreading risk in Lishui,but the human avian influenza（H7N9） virus were not found in crowd and poultry.