多重PCR和单重PCR在绍兴地区儿童流感病毒检测中的比较与应用

Comparison and application of multiplex PCR and single PCR in influenza virus detection among children in Shaoxing

目的建立一种可以同时检测人类A型流感病毒,B型流感病毒及A型流感病毒H1、H3亚型的多重PCR技术,并与单重PCR技术进行比较与应用。方法根据人类流感病毒的特异性基因设计并合成多对特异性引物,建立可同时扩增出人类流感病毒亚型特异性片段的多重PCR的方法。在对病毒的检测中与单重PCR进行特异性和灵敏度的比较。结果单重PCR对A型流感病毒检测灵敏度是44.68%(21/47),特异度是99.70%(686/688);对B型流感病毒检测的灵敏度是18.18%(2/11),特异度是97.38%(670/688)。多重PCR法对A型流感病毒检测灵敏度是44.68%(21/47),特异度是97.38%(670/688);对B型流感病毒检测的灵敏度是18.18%(2/11),特异度是97.67%(672/688)。结论本研究建立多重PCR可以准确检测流感病毒,而且所用时间短,可以快速得到检测结果。

Objective To establish a multiplex PCR method to detect the human influenza A virus, influenza B virus and influenza A virus H1 and H3 subtypes, and compare it with the single PCR. Methods The human influenza virus - specific genes were used to design specific primers. Multiplex PCR was established to amplify specific fragments of human influenza virus subtypes, and the result was compared with that of single PCR. Results The sensitivity and specificity of single - plex PCR in influenza A virus detection were 44.68% （21/47） and 99.70% （686/688） , while in influenza B virus detection were 18.18% （2/11 ） and 97.38% （670/688）. The sensitivity and specificity of muhiplex PCR method in influenza A virus detection were 44.68% （21/47） and 97.38% （670/688）, while those in influenza B virus detection were 18.18% （2/11） and 97.67% （672/688）. Conclusion Multiplex PCR was accurate and less time - consuming in influenza virus detection.