溶血标本对乙肝表面抗原ELISA法检测准确性的影响

The influence of hemolytic blood specimens on ELISA accuracy in HBsAg detection

目的观察分析不同程度溶血对乙肝表面抗原ELISA法检测的影响。方法选取健康标本50例和乙型肝炎患者标本50例,分别对正常标本和溶血后的标本采用酶联免疫吸附试验(ELISA)检测其乙肝表面抗原。结果重度溶血标本采用ELISA法检测乙肝表面抗原易对原本阴性的标本会产生假阳性干扰,但对轻微溶血和阳性标本没有明显影响。结论重度溶血易于产生对乙肝表面抗原检测的影响,应避免使用溶血标本进行乙肝表面抗原的检测。

Objective To observe and analyze the influence of hemolysis of different levels on ELISA in HBsAg detection. Methods Blood specimens were collected from 50 healthy people and 50 hepatitis B patients to detect the HBsAg by ELISA, then the detection results of normal blood specimens and hemolytic blood specimens at different hemolytic degrees were analyzed and compared. Results HBsAg of the severe hemolytic specimens were susceptible to show false positive in negative specimens, but no obvious influence was found in those with mild hemolysis and positive specimens. Conclusion Severe hemolysis can easily influence the HBsAg detection results, so it should be avoided in HBsAg detection.