RANTES基因-28C/G单核苷酸多态性与子宫内膜异位症遗传易感性的研究

Association of RANTES gene-28C/G single nucleotide polymorphism with the susceptibility to endometriosis

目的探讨调节正常T细胞表达和分泌活性因子(regulated on activation,normal T cell expressed and secreted,RANTES)基因启动子区-28C/G单核苷酸的多态性与广东籍汉族患者子宫内膜异位症的关系。方法应用聚合酶链反应-限制性酶切片段长度多态性技术(PCR-RFLP)并进行基因测序的方法检测广东籍汉族子宫内膜异位症患者59例(内异症组),非子宫内膜异位症患者49例(对照组),比较分析各组间基因型频率和等位基因频率。结果 RANTES基因启动子区-28C/C基因型在子宫内膜异症组及对照组分布频率分别为81.36%、81.63%,C/G基因型分布频率分别为18.64%、18.37%;两组间的基因型分布频率比较差异无显著性(P>0.05)。RANTES基因启动子区-28位点C等位基因型在内异症组及对照组中的分布频率分别为90.68%、90.82%,G等位基因型分布频率分别为9.32%、9.18%,两组间等位基因型频率比较差异无统计学意义(P>0.05)。结论在广东籍汉族妇女中,RANTES基因启动子区-28C/G单核苷酸多态性与子宫内膜异位症遗传易感性可能无关联。

Objective： To investigate the association of RANTES gene -28C/G single nucleotide polymorphism with endometriosis in Guangdong Hans women. Methods ： PCR - PFLP technique and gene sequencing method was used to detect C/G single nucleotide polymorphisms of - 28 locus in RANTES gene of 59 Guangdong Hans women with endometriosis （ endometriosis group） and 49 women without endometriosis （control group）. The genotype frquency and allele frlequeney were compared between the two groups. Results： The distribution frequencies of C/C genotype of -28 locus in RANTES gene were 81.36% and 81.65% in endometriosis group and control group, respectively; C/G genotype were 18. 64% and 18. 37%, respectively, there was no significant difference between the two groups （ P 〉 0. 05 ）. The distribution frequencies of C allele of - 28 locus in RANTES gene were 90.68% and 90. 82% in endometriosis group and control group, respectively, G allele were 9.32% and 9. 18%, respectively, there was no significant difference between the two groups （ P 〉 0.05）. Conclusions ： C/G single nucleotide polymorphism of - 28 locus in RANTES gene may be independent of hereditary susceptibility of endometriosis in Guangdong Hans women.