摘要
目的比较经典的酚/氯仿法和人全血基因组DNA小量快速提取试剂盒抽提法两种方法提取人全血基因组DNA的纯度及总量的差异。方法收集静脉血5毫升,共132人份。分别采用上述两种方法提取基因组DNA,用紫外分光光度仪及琼脂糖凝胶电泳检测其纯度和总量,采用统计学t检验,数据以均数±标准差(x±s)表示。结果经典的酚/氯仿法和人全血基因组DNA小量快速提取试剂盒抽提法两种方法提取基因组DNA纯度用OD260/OD280表示分别为:1.65+0.12,1.86+0.15。基因组DNA总量分别为28.3+3.02,33.8+3.24。结论从提取的基因组DNA纯度和总量来比较,人全血基因组DNA小量快速提取试剂盒抽提法抽提方法较好。
Objective： To compare the different purity and yield of human genomic DNA extracted from microsamples of whole blood by the methods of phenol/chloroform method, human whole blood genomie DNA extraction kit for fast extraction. Methods： 132 samples of 5ml peripheral venous blood were collected, and genomic DNA was extracted from them by the two methods ahead respectively. Then the purity and yield were measured by extracted from them aganose gel electrophoresis. The results were described as x ＋ s analysed with t - test. Results： DNA purity was indicated by OD260/OD280 method of phenol/chloroform method （1.65 ＋ 0. 12）, method of human whole blood genomic DNA extraction kit for fast extraction （ 1.86 ＋ 0. 15 ）. Total genomic DNA respectively were 28. 3 ＋ 3.02, 33.8 ＋ 3.24. Conclusions ： Genomic DNA extracted from the purity and amount to compare, kit extraction method was better.
出处
《中国优生与遗传杂志》
2013年第12期59-60,共2页
Chinese Journal of Birth Health & Heredity
