见血青化学成分研究

Chemical constituents from Liparis nervosa

目的研究见血青Liparisnervosa的化学成分。方法采用大孔树脂、硅胶、MCIGel、ODS以及SephadexLH-20等柱色谱方法进行分离和纯化，通过理化性质和波谱数据鉴定化合物的结构。结果从见血青75％乙醇提取物中分离得到9个化合物，分别鉴定为（六氢-1H-双吡咯烷-1-基）甲基-2，2-二甲基-8-（3-羟基异戊烷）苯并二氢吡喃-6-羧酸酯（1）、木栓酮（2）、α-菠甾醇（3）、对羟基苯甲醛（4）、当药黄素（5）、芹菜素．6，8-二-C-α-L-阿拉伯糖苷（6）、芫花素-6，8-二-C-α-L-阿拉伯糖苷（7）、芹菜素-8-C-α-L-阿拉伯糖苷（8）、胸腺嘧啶脱氧核苷（9）。结论化合物1为新的吡咯里西啶生物碱，命名为脉羊耳兰碱B。化合物2～9为首次从见血青中分离得到。

Objective To study the chemical constituents from Liparis nervosa. Methods The column chromatography on silica gel, MCI gel, ODS, and Sephadex LH-20 columns was applied for the isolation and purification of the chemical constituents. The structures were identified based on physicochemical property and spectral data. Results Nine compounds were isolated and identified as （hexahydro-lH-pyrrolizin-l-yl） methyl-2, 2-dimethyl-8-（3-hydroxyisoamyl） chroman-6-carboxylate （1）, friedelin （2）, cL-spinasterol （3）, p-hydroxybenzaldehyde （4）, swertisin （5）, 6, 8-di-C-α-L-arabinopyranosyl apigenin （6）, 6, 8-di-C-α-L-arabinopyranosyl genkwanin （7）, 8-C-α-L-arabinopyranosyl apigenin （8）, and thymidine （9）. Conclusion Compound 1 is a new compound named nervosine B, and compounds 2-9 are isolated from L. nervosa for the first time.