黑老虎化学成分及其抗氧化活性研究

Chemical constituents and their anti-oxidative activiities of Kadsura coccinea

目的研究黑老虎Kadsuracoccinea的化学成分及其体外抗氧化活性。方法利用硅胶柱色谱等方法分离纯化，结合理化性质以及光谱数据鉴定化合物结构，DPPH法检测体外清除自由基能力。结果从黑老虎95％乙醇提取物的醋酸乙酯萃取部位分离得到14个化合物，分别鉴定为micrandilactoneC（1）、3，4-二甲氧基苯基-α-D-吡喃葡萄糖苷（2）、红景天苷（3）、南五味子木脂素N（4）、原儿茶酸（5）、莽草酸乙酯（6）、莽草酸（7）、5-O-β-D-吡喃葡萄糖基龙胆酸（8）、丁香酸（9）、邻苯二甲酸二正丁基酯（10）、苯甲醇-O-β-D-吡喃葡萄糖苷（11）、4-O-β-D-吡喃葡萄糖基香草酸（12）、丁香醛（13）和莽草酸甲酯（14）。化合物5、6和14具有一定的抗氧化作用。结论化合物2、3、6、8-14为首次从该植物中分离得到；化合物5、6和14在天然抗氧化剂研发中具有一定的开发前景。

Objective To study the chemical constituents and their anti-oxidative activities of Kadsura coccinea. Methods The compounds were isolated and purified by column chromatography. The structures were determined by means of physicochemical properties and spectroscopic analyses. The anti-oxidative activity was studied by DPPH methods. Results Fourteen compounds were separated and identified as micrandilactone C （1）, 3, 4-dimethoxy phenyl-α-D-glucopyranoside （2）, salidroside （3）, schisanhenol （4）, protocatechuic acid （5）, ethyl shikimate （6）, shikimic acid （7）, 5-O-β-D-glucopyranosylgentisic acid （8）, syringic acid （9）, 1, 2-benzenedicarboxylic acid-dibuty ester （10）, benzyl-O-β-D-glucopyranoside （11）, 4-O-β-D-glucopyranosylvanillic acid （12）, syringaldehyde （13）, and methyl shikimate （14）. Compounds 5, 6, and 14 had the anti-oxidative activities. Conclusion Compounds 2, 4, 6, and 8-14 are firstly isolated from K. coccinea. Compounds 5, 6, and 14 have the certain prospects in the development of natural anti-oxidative agents.