车前子指纹图谱研究及其2种指标成分的测定

Fingerprint of Plantaginis Semen and determination of its two index contents

目的建立不同产地车前子药材的高效液相色谱指纹图谱并测定京尼平苷酸和毛蕊花糖苷2种指标成分的量。方法Zorbax sbaq-C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.5%的醋酸水溶液,体积流量1.0 mL/min,检测波长254 nm,采用梯度洗脱,建立车前子HPLC指纹图谱。结果建立了车前子的指纹图谱,确立13个共有峰,京尼平苷酸和毛蕊花糖苷质量分数均值为0.794 3%和0.664 2%。结论本研究建立的指纹图谱与指标成分测定相结合分析方法快速,可用于评价车前子的质量,并为下一步建立车前子配方颗粒的质量控制方法提供依据。

Objective To establish the fingerprint of Plantaginis Semen and to determine the two index contents of geniposidic acid and verbascoside. Methods The chromatographic separation was performed on a C18 column （250 mm × 4.6 mm, 5 μm）. The mobile phase was a mixture of acetonitrile-0.5% acetic acid aqueous solution in gradient elution. The flow rate was 1.0 mL/min, and the detection wave length was 254 nm. Similarity evaluation system was used in data analysis. Results The HPLC fingerprint of Plantaginis Semen showed 13 characteristic peaks with high similarity, and contents of geniposidic acid and verbascoside were 0.794 3%and0.664 2%, respectively. Conclusion The method that combining the determination of index contents with fingerprint analysis is timesaving and could be used for the quality control of Plantaginis Semen. It could provide a reference for the further research on the quality control of formular granule with Plantaginis Semen extracts.