胸腺肽对慢性阻塞性肺病患者树突状细胞的免疫调节

Immunoregulation of Thymosin on Dendritic Cells in Patients with COPD

目的:探讨胸腺肽对慢性阻塞性肺病(COPD)患者树突状细胞的免疫调节作用。方法:分离COPD患者和健康者外周血单个核细胞并诱导成树突状细胞,在体外通过胸腺肽干预树突状细胞分为四组,分别为COPD胸腺肽刺激组、COPD组、健康者胸腺肽刺激组、健康组,通过ELISA方法检测各组外周血树突状细胞分泌Th1型细胞因子IFN-γ和Th2型细胞因子IL-4的表达水平。结果:COPD胸腺肽刺激组较COPD组和健康者胸腺肽刺激组IFN-γ明显升高(分别P<0.05和P<0.01),而COPD胸腺肽刺激组较COPD组IL-4的表达明显降低(P<0.05),COPD组较健康组分泌细胞因子也明显增多(P<0.01)。结论:胸腺肽可调节COPD患者IFN-γ、IL-4的分泌表达,有利于改善COPD患者的免疫功能。

Objective： To investigate immune regulation of Thymosin on dendritic cells from patients with COPD. Methods：We isolated peripheral blood mononuclear cells of patients with COPD and healthy donors and transferred them into dendritic cells. These ceils were divided into four groups, which contained thymosin-stimulated dendritic cells and non-thymosin-stimulated dendritic cells respectively from patients with COPD and healthy donors. The levels of Thl cytokines IFN-＇y and Th2 cytokines IL-4 were examined from peripheral blood dendritic cells of four groups using ELISA.Results：The IFN-γ level from thymosin-stimulated dendritic ceils of patients with COPD was increased compared to non-thymosin-stimulated dendritic cells of patients with COPD and thymosin-stimulated dendritic cells of healthy donors （respectively P〈0.05 and P〈0.01）,but the IL-4 level from thymosin-stimulated dendritic cells of patients with COPD was decreased compared to non-thymosin-stimulated dendritic cells of patients with COPD （P〈 0.05）. The level of cytokines from non-thymosin-stimulated dendritic cells of patients with COPD was increased compared to healthy donors. Conclusion： This study indicates that thymosin can regulate the levels of IFN-γ and IL-4 from patients with COPD in vivo, it is beneficial to improve the immune function of patients with COPD.