谷胱甘肽在小鼠卵母细胞玻璃化冷冻中的作用

The Effect of GSH Treatment on Viritication Mouse Oocytes

目的:观察谷胱甘肽在小鼠玻璃化冷冻中的保护性作用。方法:通过卵母细胞是否玻璃化冷冻及是否添加GSH处理,将小鼠卵母细胞分为4组。检测卵母细胞内GSH浓度、ROS水平,以及通过彗星实验量化OTM值检测DNA碎片的生成。结果:在对照组、冷冻组、GSH处理组和GSH处理冷冻组细胞内GSH浓度分别为8.95±1.26、4.36±0.96、9.27±1.05和8.18±0.89;ROS水平分别为47.5±4.23、64.2±5.69、44.5±3.25 and 49.9±7.62。通过GSH处理,玻璃化冷冻卵母细胞出现彗尾百分比显著低于未处理组,差异具有统计学意义;通过GSH处理,玻璃化冷冻卵母细胞OTM值低于未处理组,差异无统计学意义。结论:玻璃化冷冻使小鼠卵母细胞产生一定的氧化应激损伤,表现为细胞内GSH浓度下降,ROS水平上升,DNA碎片增加,GSH处理可以在一定程度上改善。

Objective： To observe the protective effect of GSH on mouse oocytes after vitrification. Methods： All the mouse oocytes were divived into four groups by GSH treatment and vitrification of oocytes or not. The intracellular GSH concentration and ROS level were detected,DNA fragmentation were quantified and expressed as olive tail moment （OTM）. Results： Intracellular GSH concentration were8.95±1.26, 4.36± 0.96, 9.27± 1.05 and 8.18± 0.89 in control, vitrification, GSH treatment and GSH treatment vitrification groups respectively.Intracellular ROS level were 47.5± 4.23, 64.2± 5.69, 44.5±3.25 and 49.9± 7.62 in control, vitrification, GSH treatment and GSH treatment vitrification groups respectively. After GSH treatment, the appearance of comet tail in vitrification oocytes were statistically lower（53.4%vs36.7%）, the OTM values were lower （7.61 ± 1.91 vs 5.39± 1.21）, but there was no statistical difference. Conclusion： Oxidative stress injury were appeared in vitrification mouse oocytes, as the intracellular GSH concentration was descended and ROS level was ascended, but GSH treatment can alleviate it to a certain degree.