白细胞介素1α基因多态性与颅内动脉瘤的关联

Association between Interleukin-1α Polymorphisms and Risk of Intracranial Aaneurysm

目的探讨白细胞介素1α(IL-1α)基因多态性与颅内动脉瘤发生的关系。方法以2010年6月-2012年3月145例颅内动脉瘤患者和181例正常对照者为研究对象,采用聚合酶链反应-限制性片段长度多态性检测IL-1α-889C/T和+4845G/T多态性,统计分析基因多态性与颅内动脉瘤的相关性。结果 IL-1α-889C/T位点:CT/TT基因型在颅内动脉瘤组中的频率为32.4%,显著高于其在对照组中的频率(21.5%),两组相比差异有统计学意义(χ2=4.90,P<0.05);T等位基因在颅内动脉瘤组中的频率为16.9%,显著高于其在对照组中的频率(10.8%),两组相比差异有统计学意义(χ2=5.17,P<0.05)。IL-1α+4845G/T多态性在两组人群中的分布差异无统计学意义(P>0.05)。结论 IL-1α-889C/T多态性与颅内动脉瘤的发病有关,-889T等位基因可能是颅内动脉瘤的遗传易感基因。

Objective To investigate the association between interleukin-1α （IL-1α） polymorphisms and risk of intracranial aneurysm （IA）. Methods The IL-1a-889C/T and ＋4845G/T polymorphisms were genotyped in 145 patients with 1A and 181 healthy controls （between June 2010 and March 2012） using polymerase chain reaction-restriction fragment length polymorphism strategy. Results The frequency of IL-1a-889CT/TT genotypes was 32.4% in patients with IA and 21.5% in control subjects. The IL-1a-889CT/TT genotypes were associated with a significantly increased risk of IA compared with the CC genotype （;（2=4.90, P 〈 0.05）. The frequency oflL-1α-889T allele was 16.9% in patients with IA and 10.8% in control subjects. The IL-1a-889T allele was associated with a significantly increased risk of IA compared with the C allele （2＇2=5.17, P 〈 0.05）. There was no significant difference in the distribution of the IL-1α＋4845G/ T polymorphism between cases and controls （P 〈 0.05）. Conclusion IL-1a-889C/T polymorphism is related to the risk of IA, suggesting that -889T allele may be a susceptibility gene for IA.