哮喘患者血清巨噬细胞炎症蛋白-1α的表达及其临床意义

Expression and clinical significance of serum macrophage inflammatory protein-1 alpha of asthma

目的 观察哮喘患者巨噬细胞炎症蛋白-1α（MIP-1α）在血清中的表达,探讨其与哮喘严重度的关系.方法 选取诸暨市中心医院2012年3～10月确诊为哮喘的患者80例,根据病程不同分为A组哮喘发作期患者（40例）,B组哮喘缓解期患者（40例）,另选取同期C组40例健康者作为对照.测定各组研究对象MIP-1α浓度、外周血嗜酸性粒细胞计数（EOS）和第1秒用力呼气容积（FEV1）占预计值百分比.结果 ①A组血清MIP-1α表达水平为（51.2±6.4）ng/L,明显高于B组的（23.5±3.7）ng/L（t=23.698,P=0.000）及C组（15.1±2.8）ng/L（t=32.683,P=0.000）;与C组比较,B组MIP-1α水平明显升高（t=11.450,P=0.000）.②在外周血EOS计数的比较中,A组为（0.51±0.34）×109/L,B组为（0.26±0.19）×109/L,均显著高于C组的（0.13±0.08） ×109/L（tac=11.658,Pac=0.000;tac=3.988,Pbc=0.000）;与B组比较,A组明显升高（t=4.059,P=0.000）.③FEV1占预计值百分比的比较中,A组为（53.7±17.6）％,B组为（68.2±16.3）％均显著低于C组[（88.4±12.5）％]（tac=10.166,Pac=0.000;tbc=6.220,Pbc=0.000）;与B组比较,A组明显降低（t=3.823,P=0.000）.④A组患者血清MIP-1α浓度与外周血EOS计数之间呈正相关（r=0.422,P＜ 0.01）;与FEV1占预计值百分比呈负相关（r=-0.339,P＜0.05）.而B组患者血清MIP-1α浓度与外周血EOS计数及FEV1占预计值百分比之间均无显著相关关系（均P＞ 0.05）.结论 哮喘发作期时血清MIP-1α表达水平明显增高,与外周血EOS计数和FEV1占预计值百分比之间存在明显的相关关系,监测血清MIP-1α的水平对哮喘具有一定的诊断价值.

Objective To observe the expression and clinical significance of serum macrophage inflammatory protein-1 alpha （MIP-1α） of asthma.Methods 80 patients with asthma in the Central Hospital of Zhuji City from March to October in 2012 were selected and divided into two groups by different process of the disease.40 onset asthmatic patients were devided into group A,the other 40 paracmastic asthmatic patients were devided into group B,another 40 health individuals were collected as group C （control group）.The levels of MIP-1α,the number of eosinophilic granulocyte （EOS） and the percentage of FEV1 in prediction among three groups were measured.Results ①The level of MIP-1α in group A [（51.2±6.4） ng/L] was higher than in group B [（23.5t3.7） ng/L] and group C [（15.1±2.8） ng/L] （tab =23.698,Pab =0.000; tac =32.683,Pac =0.000）.The level of MIP-1α in group B was higher than that in group C （t =11.450,P =0.000）.②The number of EOS in group A [（0.51 ±0.34）×109/L] and in group B [（0.26±0.19）×109/L] were both higher than in group C [（0.13±0.08）×109/L] （tac=11.658,Pac =0.000; tbc =3.988,Pbc =0.000）.The number of EOS in group A was higher than in group B （t =4.059,P =0.000）.③The level of the percentage of FEV1 in prediction in group A [（53.7±17.6） ％] and in group B [（68.2±16.3） ％] were both lower than in group C [（88.4±12.5） ％] （tac =10.166,Pac =0.000; tbc =6.220,Pbc =0.000）.The level of the percentage of FEV1 in prediction in group A was higher than that in group B （t =3.823,P =0.000）.④There was positive correlation was found between MIP-1α and the number of EOS in group A （r =0.422,P ＜ 0.01）; the negative correlation was found between MIP-1α and the percentage of FEV1 in prediction in group A （r =-0.339,P ＜ 0.05）.No correlation between MIP-1α and the number of EOS was found,no correlation between MIP-1α and the percentage of FEV1 in prediction was found （all P ＞ 0.05）.Conclusion The level of MIP-1α increases in period of onset asthma,and has correlation with the number of EOS and the percentage of FEV1 in prediction.It has a certain value to monitor the level of MIP-1α in serum.