摘要
用溶葡球菌酶基因的克隆菌株枯草杆菌Bacillus subtilis 0044作为生产菌,研究了该菌的生长和产酶条件。结果表明:该菌株能利用国产廉价培养基有效地合成和分泌溶葡球菌酶,产量达200mg/L培养液;添加抗菌素能维持质粒的稳定及保证溶葡球菌酶的产量;添加表面活性剂Tween-80可使酶产量提高20%～40%;产酶高峰期为12～15h,16 h以后,溶葡球菌酶产量迅速下降,这种产量下降的现象似与蛋白酶活性的升高有关;在适宜时期添加葡萄糖,可使产酶高峰期延长。
This paper deals with the fermentation of lysostaphin. A cloned B. subtilis
strain could secretelysosta phin efficiently into the medium with a yield of about 200
mg/ml of medium by using cheap home-made products, antibiotic selection was
necessary for the plasmid stability, and lysostaphin production; yield was increa-
sed by 20%~40% in the presence of surfactant; maximum production was obtained
at about 14 hours of incubation. The decrease of lysostaphin showed that it was related
to the increase of protease activity. The addition of glucose at right time prolong
the longterm production of lysostaphin.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1991年第3期293-296,共4页
Journal of Fudan University：Natural Science
关键词
溶葡球菌酶
抗菌素
发酵
葡萄糖
fermentation, antibiotics, glucose
lysostaphin.
