螺旋藻提取物对表皮生长因子受体酪氨酸激酶的抑制作用及诱导HL-60细胞凋亡

Inhibitory effect of spirulina extract on EGFR tyrosine kinase and its ability to induce apoptosis in HL-60 cells

目的： 研究螺旋藻提取物 (藻福康 )对肿瘤细胞生长的抑制作用。 方法： 运用 ELISA法检测藻福康对表皮生长因子受体 (epidermal growth factor receptor,EGFR)酪氨酸激酶的作用；用荧光显微镜、流式细胞术和琼脂糖凝胶电泳分析细胞凋亡。 结果： 藻福康能明显抑制 EGFR酪氨酸激酶的活性， IC50为 0.125mg/ml；不管是细胞形态分析，还是流式细胞仪检测或 DNA片段电泳，藻福康都显示能诱导 HL 60细胞凋亡。 结论： 藻福康能明显抑制 EGFR酪氨酸激酶的活性和诱导白血病细胞凋亡。

Objective: The current study was designed to investigate anticancer mechanism of Zaofukang, the extract of spirulina platensis. Methods: The effect of Zaofukang on epidermal growth factor receptor (EGFR) protein tyrosine kinase was determined by using ELISA assay. Cell apoptosis was evaluated by using fluorescence microscopy, flow cytomery and agarose gel electrophoresis. Results: Zaofukang inhibited EGFR tyrosine kinase activity directly with its IC50 of 0.125 mg/ml, and also induced apoptosis of human leukemia cells(HL 60). Conclusion: These results suggest that Zaofukang could inhibit the activity of EGFR protein tyrosinen kinase and induce apoptosis in HL 60 cells.