柑桔溃疡病菌离体叶接种检验法的研究

Detection Method of Inoculation on Citrus Leaves in vitro with Xanthomonas axonopodis pv. citri

对柑桔溃疡菌的离体叶接种检验法进行了研究和技术规范。结果表明 ,离体叶接种法是一种效果直观可靠、操作简便易行的检验溃疡病菌的方法 ,其检测水平可达到 1.5 5× 10 3 -4 cfu/ml;其技术规范为 :供接种的柑桔品种应为甜橙类感病品种 (如脐橙 ) ,供接种的离体叶片以达到定型大小的新梢成叶最佳 ;采用针刺后滤纸片贴敷菌液接种 ,最适孵育温度为 2 8～ 32℃ ,最佳保湿方法为吸水纸保湿法。

Detection method of inoculation with Xanthomonas axonopodis pv. citri on citrus leaves in vitro is studied and standardized in this paper. The results indicate that the established method is simple and practical, in which detection level of 1.55×10\+\{3～4\}cfu/ml can be reached. The following conditions are the optimum: mature leaves on each new shoots of susceptible citrus cultivar must be inoculated; the incubation temperature had better to be controlled between 28～32℃, and humidity has to be kept stable with blotter method after needle punctured inoculation.\;