狼亚种分化的RAPD分子证据

Molecular Evidence of RAPD in Wolf Subspecies Differentiation

对来自我国东北、内蒙、甘肃、新疆等１０个地区的２１ 只狼（ Ｃａｎｉｓ ｌｕｐｕｓ）应用 ＲＡＰＤ方法进行亚种分化研究．筛选出２５个随机引物。通过对ＲＡＰＤ产生的谱带进行比较，找出共享片段．计算共享度和遗传ＢＥ离。并进行聚类分析，做出聚类图，确定亲缘关系和分类地位。研究结果表明：（１）中国南北方狼有分化，在０ＮＡ序列上存在着明显差异；（２）把所研究地区的狼划分为５个亚种．即：云贵群体（云南群体和贵州群体）和东南群体（福建群体、浙江群体和广东群体）亚种；东北群体、河北群体亚种：甘肃群体亚种：内蒙群体亚种：新疆群体亚种。

solves (Canis lupus). which came from 10 areas in China, Northeast area, Inner Mongolia area. Gansu area and Xinjiang area, were studied using random amplified polymorphic DNA(RAPD) markers. 25 primers were screened and RAPD bands were obtained. the shared fragments and genetic distances were calculated by studying the electrophoresis patterns of RAPD. The results showed that the DNA orders of wolves in tile south and north of China exist apparently differences. The wolves in the research areas were divided into 5 subspecies. Yungui and Southeast populations, Northeast and Hebei populations, Gansu pupulation. Inner. mongolia population and Xinjiang populations. Yungui population includes Yunan and Guizhou populations. and Southeast population includes Fujian. Zhejiang and Guangdong populations.