γ-干扰素对溶藻弧菌(Vibrio alginolyticus)在青石斑鱼(Epinephelus awoara)巨噬细胞胞内存活的影响

EFFECTS OF IFN-GAMMA ON INTRACELLULAR SURVIVAL OF VIBRIO ALGINOLYTICUS IN MACROPHAGES OF EPINEPHELUS AWOARA

采用大肠杆菌重组γ-干扰素(rIFN-gamma)单独和联合处理青石斑鱼(Epinephelus awoara)头肾巨噬细胞的方法,进行了重组γ-干扰素对溶藻弧菌(Vibrio alginolyticus)在巨噬细胞内存活研究。结果表明,溶藻弧菌感染巨噬细胞后暴露于-干扰素的细胞内细菌存活数量[(1380±80)CFU/mL]明显降低。然而,在24h,γ-干扰素及脂多糖联合处理组巨噬细胞内溶藻弧菌的存活数量[(463±32)CFU/mL]比暴露于含有或者不含有脂多糖,NG-单甲基-L-精氨酸(NMMA)和过氧化氢酶的-干扰素处理组少。采用γ-干扰素,γ-干扰素和脂多糖,γ-干扰素、脂多糖和NMMA,γ-干扰素、脂多糖和过氧化氢酶四种方式处理24h能提高巨噬细胞过氧化氢的释放,而不能提高NO2的产物。另外,经过-干扰素处理能够明显降低巨噬细胞对溶藻弧菌的的吞噬作用,但不影响对溶藻弧菌的杀伤作用。本文数据表明,过氧化氢的释放和细胞吞噬作用能明显减少溶藻弧菌在巨噬细胞内存活的数量。

The effects of recombinant Escherichia coli gamma interferon （rIFN-gamma） on the interaction of Vibrio alginolyticus with Epinephelus awoara head kidney were examined. The results show that the number of intracellular survival V. alginolyticus in macrophages exposed to IFN-gamma after infection with V. alginolyticus was fewest [（1380± 80）CFU/mL] at 24 hours among the four treatment ways. However, the number of intracellular survival V. alginolyticus in macrophages treated with INF-gamma and in combination with LPS gained the fewer [（463±32）CFU/mL] at 24 hours than that in macrophages exposed to INF-gamma together with or without LPS and NMMA and catalase. Macrophages treated with IFN-gamma or together with LPS or LPS and NMMA or LPS and catalase enhanced the release of H2O2 at 24 hours, while these did not reinforce the NO2- production. What’s more, macrophage treated with IFN-gamma significantly reduced phagocytosis of V. alginolyticus, while this didn’t cause influence to killing of V. alginolyticus. Our data demonstrated that the amount of H2O2 release and phagocytosis can contribute to the decrease in intracellular V. alginolyticus.