海洋脂肪酶ADM47601固定化方法的研究

RESEARCH ON THE IMMOBILIZATION OF LIPASE ADM47601

采用海藻酸钠、壳聚糖、聚乙烯醇和明胶等材料,进行对脂肪酶ADM47601的固定化研究。结果表明,使用壳聚糖固定化脂肪酶,在最优条件为2%(W/V)壳聚糖,10%NaOH,1%乙酸,0.25%戊二醛,每克载体添加840U脂肪酶时,最大固定化酶活力回收率为87.06%。使用海藻酸钠-明胶固定化脂肪,在最优固定化条件下,最大固定化酶活力回收率为54.45%。使用聚乙烯醇固定化脂肪酶,在最优固定化条件下,最大固定化酶活力回收率为33.22%。使用海藻酸钠固定化脂肪酶,在最优固定化条件下,最大固定化酶活力回收率为17.11%。对比四种不同固定化酶方法,脂肪酶活力回收率高度高低顺序为:壳聚糖吸附交联法>海藻酸钠明胶协同包埋法>聚乙烯醇-硼酸法>海藻酸钠包埋法。

We treated marine lipase ADM47601 with sodium alginate, mixture of sodium alginate and gelatin, chitosan, and polyvinyl alcohol to study the immobilization properties of the lipase. The results are： （1） immobilization with sodium alginate, at the optimal conditions of 3% sodium alginate, 1% CaCl2, 600U/mL lipase addition, the maximum immobilized lipase activity recovery rate was 17.11%; （2） with the mixture of sodium alginate and gelatin, at 1% alginate, 0.5% CaCl2, 0.25% glutaraldehyde, 600U/mL lipase addition, the maximum recovery rate was 54.45%; （3） with chitosan, at 2% chitosan, 10% NaOH, 1% acetic acid, 0.25% glutaraldehyde, 840U/g lipase addition, the maximum rate could reach 87.06%; and （4） with polyvinyl alcohol, at conditions of 7.5% polyvinyl alcohol, 2% sodium alginate, 1.5% CaCl2, and 360U/mL lipase addition, the maximum rate was 33.22%. Therefore, among the four materials, chitosan is the best for the highest recovery, followed by sodium-alginate-gelatin mixture, polyvinyl alcohol, and sodium alginate.