痰瘀同治方对冠心病痰瘀互结证小型猪模型中医证候评分的影响

Effect of formula of removing both phlegm and blood stasis on Chinese medicine symptom complex score for coronary heart disease Chinese miniature swine model with phlegm-stasis cementation syndrome

目的:建立冠心病痰瘀互结证小型猪模型"拟临床"中医证候评分方法,观察痰瘀同治方对冠心病痰瘀互结证小型猪模型中医证候评分的影响。方法:将36只中国小型猪随机分为正常对照组、模型组、舒降之组、痰瘀同治方高、中、低剂量组(生药2.0,1.0,0.5 g·kg-1),每组6只;除对照组外,其他各组高脂饲料喂养2周后,采用介入技术球囊损伤冠状动脉左前降支内皮,术后继续高脂饲料喂养8周,制备小型猪痰瘀互结证冠心病模型;术后给药8周,根据症状分级评分方法,分析各组动物冠心病痰瘀互结证主症、兼症、舌象和脉象评分的变化。结果:与对照组比较,模型组在高脂6周和10周时,主症、兼症、舌象和脉象评分均明显升高(P<0.05或P<0.01);与模型组比较,痰瘀同治方不同剂量均能够降低6周和10周时主症评分;此外低剂量还能降低10周时舌象和脉象评分;中剂量能降低6周时舌象评分和10周时兼症、舌象和脉象评分;而高剂量则降低6周和10周时各项评分(P<0.05或P<0.01)。结论:痰瘀同治方能够改善冠心病痰瘀互结证小型猪模型主症、兼症、舌象和脉象评分,提示"拟临床"小型猪病证客观化评分对于中药新药研发具有重要意义。

Objective： To establish the ＂clinical-mimetic＂ Chinese medicine symptom complex score method for the coronary heart disease Chinese miniature swine model with phlegm-stasis cementation syndrome, in order to observe the effect of formula of removing both phlegm and blood stasis （TYTZ） on the Chinese medicine symptom complex score for the coronary heart disease Chinese miniature swine model with phlegm-stasis cementation syndrome. Method： Totally 36 Chinese miniature swine were randomly divided to the normal control group, the model group, the Shujiangzhi group, and TYTZ groups with doses of 2. 0, 1.0 and 0. 5 g · kg^-1 , with six in each group. Except for the normal control group, all of the other groups were fed with high fat diet for two weeks. The coronary heart disease model with phlegm-stasis cementation syndrome was established by injuring left anterior descending artery with interven- tional balloons and continuously feeding with high fat diet for eight weeks. After the operation, the groups were administered with drugs for eight weeks. Their main symptoms, accompanied symptoms, tongue and pulse signs of the coronary heart disease Chinese miniature swine with phlegm-stasis cementation syndrome were observed according to the symptom-graded scoring method. Result： Compared with the model group, TYTZ in different doses could reduce the scores of main symptoms at the 6th and 10th week. Specifically, TYTZ in low dose could reduce the scores of tongue at the 6th week and the scores of accompanied symptoms, and tongue and pulse signs at the 10th week; And TYTZ in high dose could decrease all symptom scores at the 6th and 10th week （P 〈 0. 05 or P 〈 0.01 ）. Conclu- sion ： TYTZ can imlprove the scores of the main symptoms, accompanied symptoms, tongue and pulse signs in coronary heart disease Chinese miniature swine with phlegm-stasis cementation syndrome. It is suggested that the ＂clinical-mimetic＂ objective scoring for syndromes of Chinese miniature swine is of great significant to the development of new traditional Chinese medicine drugs.