氟西汀干预对抑郁大鼠模型脑脊液促炎性细胞因子水平的影响

Effect of fluoxetine intervention on the level of pro-inflammatory cytokines in CSF of rat depressive model

目的探讨氟西汀干预对抑郁大鼠模型脑脊液促炎性细胞因子水平的影响及意义。方法根据行为学评分挑选分数近似的20只SD大鼠进行4周的慢性不可预知应激及孤养模式相结合的建模,实验前、建模4周后及氟西汀干预4周后分别检测大鼠脑脊液中白介素(IL)-6、IL-2、IL-10水平,并进行统计学分析。结果相对于建模前,建模后大鼠脑脊液中IL-6及IL-2水平均有提高,差异有统计学意义(P<0.01),IL-10水平未见明显变化;氟西汀干预4周后,建模大鼠脑脊液中IL-6及IL-2水平较干预前减低,但未达到正常水平。结论氟西汀虽然不是抗炎药物,但能通过有效恢复正常脑细胞功能,使神经细胞分泌促炎性细胞因子减少,加快抑郁症的好转与恢复。

Objective To explore the effect and significance of fluoxetine intervention on the level of pro-inflammatory cytokines in cerebrospinal fluid （CSF） of rat depressive model. Methods Based on the evaluation of ethology, 20 SD rats were exposed to chronic unpredictable mild stress for 4 weeks and raised separately, detecting the levels of interleukin-6 （IL-6）, inter- leukin-2 （IL-2）, interleukin- 10 （IL- 10） in CSF at the beginning, 4 weeks later and 4 weeks after fluoxetine intervention. Statistical analysis was conducted on the data. Results Compared with the prior modeling,the levels of IL-6,IL-2 in CSF of depressive model rose after modeling with statistically significant differecne,but no significant change was found in the IL-10. 4 weeks after fluoxetiue intervention, the levels of IL-6,IL-2 in CSF of the rats reduced obviously, but failed to reach the normal level. Conclu- sion Although the fluoxetine is not the anti-inflammatory drug,it can reduce the pro-inflammatory ceils by restoring neurons function, accelerate the improvement and recovery of the depression.