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Effect of Static Magnetic Field on Extracellular Proteins Synthesis in Escherichia coli

Effect of Static Magnetic Field on Extracellular Proteins Synthesis in Escherichia coli
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摘要 Escherichia coli type 1 was used as a model system to determine whether static magnetic fields are a general stress factor. The bacterial broth culture were exposed to different magnetic force (400, 800, 1200 and 1600 Gauss) with incubation at 37 ℃ for different times (24, 48 and 72 hrs) under aerobic conditions. The response of the cells to the magnetic fields was estimated from the change in total protein synthesis by using spectrophotometer at 550 nm and by using of SDS-PAGE (Sodium dodecyl sulfate- polyacrylamide gel electrophoresis). Results concluded that was approximately no reproducible changes qualitatively in extracellular proteins were observed in the SDS-PAGE electrophoresis and did not act as a general stress factor. While, increases in the level of extra-cellular synthesis were observed using different magnetic field exposed samples when compared with the control.
出处 《Journal of Life Sciences》 2013年第8期796-801,共6页 生命科学(英文版)
关键词 Magnetic field optical density Escherichia coli extracellular protein electrophoresis. 蛋白合成 大肠杆菌 静磁场 SDS-PAGE电泳 聚丙烯酰胺凝胶电泳 十二烷基硫酸钠 分光光度计 细胞外
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