耐铜菌株的筛选及其对铜的去除特性

Screening of copper tolerance fungi strains and their characteristics for copper removal

采用稀释平板法,从铜污染红壤中分离得到2株耐铜真菌。对菌株的形态和ITS基因序列同源性进行分析。结果表明:2株真菌分别为淡紫色拟青霉(Paecilomyces lilacinus)和土曲霉(Aspergillus terreus),最高可耐受铜浓度均为300 mg/L。在Cu2+质量浓度为10 mg/L的培养基中添加10 g/L湿菌,在pH 5、25℃、120 r/min条件下振荡吸附30 min后,P.lilacinus和A.terreus对Cu2+的吸附率分别达85.45%和87.54%。

Two fungi strains with resistance to copper were isolated from red soil polluted by copper based on dilution plate method. The results from both morphology observation and ITS sequence analysis showed that the two fungi strains were Paecilomyces lilacinus and Aspergillus terreus. They both could endure copper concentration 300 mg/L at most. By added 10 g/L fungi strains into pH 5, temperature 25 ℃ culture medium with 10 mg/L Cu^2＋ and oscillated for 30 min with 120 r/min frequency, the removal rate of Cu^2＋ by P. lilacinus and d. terreus were 85.45% and 87.54%, respectively.