摘要
目的:研究结直肠癌中Kiss-1基因启动子甲基化状态对Kiss-1基因表达的影响。方法:应用甲基化特异性PCR(MSP)方法检测73例结直肠癌、正常结直肠组织和人结直肠癌细胞HCT116、SW480、W1116、LoVo中Kiss-1基因启动子甲基化状态,应用realtime-PCR、Western-blot技术检测相应组织和细胞中Kiss-1基因mRNA和蛋白质(Metastine)的表达量。结果:结直肠癌中Kiss-1基因甲基化阳性率(82.19%)高于正常组织(6.31%)(P<0.05);结直肠癌组织中,甲基化阳性组Kiss-1基因mRNA和Metastine表达量均明显低于甲基化阴性组(P<0.05)。而HCT116、SW116和SW480细胞中Kiss-1基因启动子甲基化阳性,LoVo细胞甲基化阴性;定量分析Kiss-1基因mRNA和matestin表达量,结果显示LoVo>SW480>SW1116>HCT116,差异有统计学意义(p<0.05)。结论:结直肠癌中Kiss-1基因启动子甲基化可能引起Kiss-1基因表达下调。
Objective：To research the effect of the Kiss-1 gene promoter methylation on Kiss-1 gene expression in colorectal carci-noma. Methods：Kiss-1 gene promotor methylation and its expression were detected respectively by methylation-specific PCR, real-time PCR and western-blot in 73 cases of colorectal carcinoma and each normal tissue. Results：The positive expression rate of Kiss-1 gene methylation in the carcinoma tissue was higher than the rate in normal tissue （82.19%vs 6.31%, P 〈0.05）. In the cancer tissue, the Kiss-1 gene mRNA and metastine expression of Kiss-1 methylation-positive group was significant lower than that in negative group （P〈0.05）. Conclusions：Kiss-1 gene promoter hyper-methylation may induce to the Kiss-1 gene expression decreased in colorectal carci-noma.
出处
《黑龙江医药》
CAS
2013年第6期1007-1010,共4页
Heilongjiang Medicine journal
基金
福建省教育厅科技计划项目(JB11054)
福建省自然科学基金课题(2013J01291)
