白花丹素镧(Ⅲ)配合物与牛血清白蛋白相互作用的光谱学研究

Spectroscopic study on the interaction between cytotoxic lanthanum(Ⅲ )complex of plumbagin with bovine serum albumin

白花丹素(Plumbagin,简称PLN)是从传统中药白花丹中提取出来的具有抗肿瘤活性的萘醌类化合物(2-甲基-5-羟基-1,4萘醌),而白花丹素镧(Ⅲ)配合物[PLN-La(Ⅲ)]对MCF-7、BEL7404和NIC-460等肿瘤细胞株具有体外细胞毒活性.采用荧光光谱、同步荧光及紫外光谱法研究了PLN和PLN-La(Ⅲ)与牛血清白蛋白(BSA)的相互作用.结果表明,PLN和PLN-La(Ⅲ)均可通过静态荧光猝灭的方式减弱BSA的荧光强度;同PLN相比,PLN-La(Ⅲ)与BSA的结合常数较小.

Plumbagin （PLN） was isolated from plumbago zeylanica, an anticancer traditional Chinese medicine. PLN and its complex with La （Ⅲ）, La （C11 H7O3 ） 3 （ H2 O） 2 , were then allowed to interact with bovine serum albumin （BSA）. The interactions among PLN and La（C11 H7O3）3 （H2O）2 and BSA were investigated by fluorescence spectrometry, synchronous fluorescence spectrometry, and ultraviolet-visible light absorption spectrometry. It was observed that PLN and La（ Ⅲ） complex can reduce the fluorescence intensity of BSA by way of static quenching. Besides, the binding constant of La（ Ⅲ ） complex with BSA is obviously lower than that of PLN with BSA.