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一株野生酵母β-葡萄糖苷酶的分离纯化 被引量：2

Isolation and Purification of β-glucosidase from Hanseniaspora uvarum
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摘要 对葡萄汁有孢汉逊酵母(Hanseniaspora uvarum)β-葡萄糖苷酶(β-glucosidase,HuBG)的分离纯化方法进行了研究。H.uvarum酵母发酵液经过硫铵沉淀、苯基疏水、离子交换、分子筛纯化后,得到电泳纯的β-葡萄糖苷酶。研究结果表明,野生酵母的β-葡萄糖苷酶在细胞中,大部分被分泌到胞外;酶的纯化倍数为34.88倍,比酶活为5.58 U/mg,SDS-page检测此酶的分子量约为80 kDa。 The isolation and the purification of β-glucosidase from Hanseniaspora uvarum in grape juice were investigated. In the experiment, H. uvarum fermenting liquid was treated by ammonium sulfate precipitation, hydrophobic interaction, ion exchange and gelfiltration chromatography to obtain HuBG through electrophoretic homogeneity. The experimental results revealed that the extracellular fraction （supematant） contained the majority of β-glucosidase and HuBG was purified to 34.88-fold with a specific activity of 5.58 U/mg. Furthermore, the molecular weight of BG1 was estimated to be 80 kDa by SDS-PAGE.
作者 王玉霞 张超
机构地区 生物工程研究所 食品科学与工程研究所
出处 《酿酒科技》 北大核心 2013年第12期42-45,共4页 Liquor-Making Science & Technology
基金 四川省教育厅重点项目<发酵型青梅保健新酒种研发>(13ZA0197) 博士启动项目<风味酶对果酒增香差异机理的研究>(2011KFZ002) 发酵与资源利用四川省重点实验室开放基金重点项目<利用柑橘果渣生产果醋饮料工艺技术研究>(2011KFZ002)
关键词 微生物 酵母 Β-葡萄糖苷酶 分离 纯化 microbe yeast β-glucosidase isolation purification
分类号 TS261.1 [轻工技术与工程—发酵工程] Q93-3 [生物学—微生物学]
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参考文献25

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二级参考文献16

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酿酒科技
2013年 第12期

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